Coat protein-mediated resistance to Turnip mosaic virus in Chinese cabbage (B. rapa ssp pekinensis) by the pollen-tube pathway

被引：0

作者：

Wei, Han Li ^{[1
]}

Yu, Ge ^{[1
]}

Lei, Rao ^{[2
]}

Shi, Cui Chong ^{[1
]}

Ping, Qu Shu ^{[1
]}

机构：

[1] Northeast Agr Univ, Dept Hort, Key Lab Biol & Genet Improvement Hort Crops North, Harbin 150030, Peoples R China

[2] Beijing Acad Agr & Forestry Sci, Beijing Agr Biotechnol Res Ctr, Beijing 100097, Peoples R China

来源：

RESEARCH JOURNAL OF BIOTECHNOLOGY | 2014年 / 9卷 / 02期

关键词：

Chinese cabbage (B. rapa ssp pekinensis); Turnip mosaic virus; TuMV-CP; Coat protein-mediated resistance; Pollen-tube pathway; FUNCTIONAL-ANALYSIS; TRANSGENIC MAIZE; EXOGENOUS DNA; MARKER-FREE; TRANSFORMATION; GENE; CASSETTE; DISEASES; COTTON; GFP;

D O I：

暂无

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

In this research we established a plant transformation system of coat protein-mediated resistance to Turnip mosaic virus. The gene TuMV-CP was introduced into Chinese cabbage through the pollen-tube pathway using the plasmid pBBBast-TuMV-CP (9.79 kb) which was composed of the gene TuMV-CP, expression regulatory sequence (CaMV 35S promoter, OCS terminator), the herbicide resistant gene Bar and other elements. A total of 0.15% of T-0 plants was positive for the gene TuMV-CP by specific PCR. Further PCR Southern blot analyses showed that TuMV-CP insertions were harbored in T-0 plants.

引用

页码：75 / 78

页数：4

共 35 条

[1] COAT PROTEIN-MEDIATED RESISTANCE AGAINST VIRUS-INFECTION [J].