Whole genome amplification of the rust Puccinia striiformis f. sp tritici from single spores

被引：5

作者：

Wang, Yanchun ^{[1
]}

Zhu, Mingqi ^{[1
]}

Zhang, Rong ^{[1
]}

Yang, Hanli ^{[1
]}

Wang, Yang ^{[1
]}

Sun, Guangyu ^{[1
]}

Jin, Shelin ^{[2
]}

Hsiang, Tom ^{[3
]}

机构：

[1] NW A&F Univ, Coll Plant Protect, Yangling, Shaanxi, Peoples R China

[2] Gansu Agr Sci Acad, Inst Plant Protect, Lanzhou, Gansu, Peoples R China

[3] Univ Guelph, Dept Environm Biol, Guelph, ON N1G 2W1, Canada

来源：

JOURNAL OF MICROBIOLOGICAL METHODS | 2009年 / 77卷 / 02期

基金：

中国国家自然科学基金; 加拿大自然科学与工程研究理事会;

关键词：

beta-tubulin gene; Multiple displacement amplification; rDNA-ITS; Single-spore; Whole genome amplification; MULTIPLE DISPLACEMENT AMPLIFICATION; PHI29; DNA-POLYMERASE; RIBOSOMAL-RNA; BETA-TUBULIN; GENE; CELL; PCR; IDENTIFICATION; POLYMORPHISM; CLONING;

D O I：

10.1016/j.mimet.2009.02.007

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Rust fungi are obligate parasites and cannot be routinely cultured to obtain sufficient biomass for DNA extractions. Multiple displacement amplification (MDA) was demonstrated in this study for whole genome amplification from single spores of the rust fungus, Puccinia striiformis. The genomic DNA coverage and fidelity of this method was evaluated by PCR amplification and sequencing of two genetic markers: portions of the multi-copy nuclear ribosomal DNA internal transcribed spacer region (ITS) and the single copy beta-tubulin gene from two geographical diverse isolates. our results show that MDA is a valuable tool for whole genome amplification from single spores, and we propose that MDA-amplified DNA can be used for molecular genetic analysis of the wheat yellow rust fungus. (C) 2009 Elsevier B.V. All rights reserved.

引用

页码：229 / 234

页数：6

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