Factors responsible for plaque formation of A/duck/Siberia/272/1998 (H13N6) influenza virus on MDCK cells

被引：10
作者：
Tsuda, Yoshimi [1 ,2 ]
Isoda, Norikazu [1 ]
Sakoda, Yoshihiro [1 ]
Kida, Hiroshi [1 ,2 ]
机构：
[1] Hokkaido Univ, Grad Sch Vet Med, Dept Dis Control,Lab Microbiol, Kita Ku, Sapporo, Hokkaido 0600818, Japan
[2] Hokkaido Univ, Res Ctr Zoonosis Control, Kita Ku, Sapporo, Hokkaido 0010020, Japan
来源：
VIRUS RESEARCH | 2009年 / 140卷 / 1-2期
关键词：
Influenza virus; Plaque formation; Fusion activity; Polymerase activity; A VIRUS; MEMBRANE-FUSION; HIGH VIRULENCE; HEMAGGLUTININ SUBTYPE; RNA-POLYMERASE; NUCLEAR EXPORT; KIDNEY-CELLS; NS1; PROTEIN; M1; BINDING;
D O I：
10.1016/j.virusres.2008.12.005
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Many influenza A viruses form plaques on Madin-Darby canine kidney (MDCK) cells in the presence of trypsin. A/duck/Siberia/272/1998 (H13N6) (Sib272), however, does not form plaque on MDCK cells. After three blind passages of the strain on MDCK cells, plaque-forming variant was obtained and designated as A/duck/Siberia/272PF/1998 (H13N6) (Sib272PF). Genetic and functional analyses of Sib272 and Sib272PF revealed that amino acid substitutions, F3L of the HA2 subunit and T379K of the PB1, were responsible for plaque formation of Sib272PF by enhancing fusion and polymerase activities, respectively. (c) 2009 Elsevier B.V. All rights reserved.
引用
收藏
页码：194 / 198
页数：5
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