Development of Direct Competitive ELISA for Residue Analysis of Fungicide Chlorothalonil in Vegetables

被引：11

作者：

Okazaki, Fumiko ^{[1
]}

Hirakawa, Yuki ^{[2
]}

Yamaguchi-Murakami, Yukie ^{[1
]}

Harada, Ayako ^{[3
]}

Watanabe, Eiki ^{[4
]}

Iwasa, Seiji ^{[3
]}

Narita, Hiroshi ^{[1
]}

Miyake, Shiro ^{[2
,5
]}

机构：

[1] Kyoto Womens Univ, Higashiyama Ku, Kyoto 6058501, Japan

[2] Adv Sci Technol & Management Res Inst Kyoto, Shimogyo Ku, Kyoto 6008813, Japan

[3] Toyohashi Univ Technol, Toyohashi, Aichi 4418580, Japan

[4] Natl Inst Agroenvironm Sci, Tsukuba, Ibaraki 3058604, Japan

[5] Horiba Ltd, Minami Ku, Kyoto 6018510, Japan

来源：

FOOD HYGIENE AND SAFETY SCIENCE | 2014年 / 55卷 / 02期

关键词：

monoclonal antibody; immunoassay; dc-ELISA; chlorothalonil; LINKED-IMMUNOSORBENT-ASSAY; PESTICIDE-RESIDUES; IMMUNOASSAY; VALIDATION; ONTARIO; FRUITS;

D O I：

10.3358/shokueishi.55.65

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

A direct competitive (dc)-ELISA was developed for rapid and simple determination of chlorothalonil residue in vegetables. A carboxylic acid derivative of pentachlorophenol was used to prepare an anti-chlorothalonil monoclonal antibody (MoAb) that showed adequate reactivity for dc-ELISA. Before homogenization of vegetable samples, phosphoric acid was added (vegetable-10% phosphoric acid (2: 1, w/v)) to block enzymatic decomposition of chlorothalonil. The use of phosphate buffer (100 mmol/L, pH 7.0) minimized the influence of phosphoric acid on competitive reaction in the dc-ELISA. Working range was 0.10 to 6.0 ng/mL in the optimized dc-ELISA. The recovery of chlorothalonil spiked in cucumber and eggplant was 97.1 to 125%. The results correlated well with those obtained by HPLC analysis. The dc-ELISA could rapidly determine chlorothalonil after a simple sample preparation procedure.

引用

页码：65 / 72

页数：8

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