Laccase activity from the fungus Trametes hirsuta using an air-lift bioreactor

被引:60
作者
Couto, S. Rodriguez [1 ]
Rodriguez, A.
Paterson, R. R. M.
Lima, N.
Teixeira, J. A.
机构
[1] Univ Rovira & Virgili, Dept Chem Engn, Tarragona 43007, Spain
[2] Univ Vigo, Dept Chem Engn, Vigo, Spain
[3] Univ Minho, Dept Biol Engn, Braga, Portugal
关键词
air-lift bioreactor; carbon source; glycerol; laccase; Trametes hirsuta;
D O I
10.1111/j.1472-765X.2006.01879.x
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Aim: To produce high laccase activities from the white-rot fungus Trametes hirsuta in an in-house air-lift bioreactor (ALB). Methods and Results: Trametes hirsuta was grown in a 6-l ALB. A fed-batch strategy with glycerol as an addition resulted in maximum laccase activity of 19 400 U l(-1), which was the highest reported from the fungus. Conclusion: The ALB configuration with additional glycerol resulted in high laccase activities. Significance and Impact of the Study: This study provides useful information on how to produce high concentrations of laccase.
引用
收藏
页码:612 / 616
页数:5
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