Salting-out effects on the partition of proteins in aqueous two-phase systems

被引：0
|
作者：
Kim, CW
Rha, CK
机构：
[1] KOREA UNIV,COLL NAT RESOURCES,DEPT GENET ENGN,SEOUL 136701,SOUTH KOREA
[2] MIT,BIOMAT SCI & ENGN LAB,CAMBRIDGE,MA 02139
来源：
JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY | 1996年 / 6卷 / 05期
关键词：
salting-out; aqueous two-phase systems; liquid-liquid extraction; PEG (polyethylene glycol);
D O I：
暂无
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
The partition of proteins in the salt-rich phase of polyethylene glycol (PEG)/salt aqueous two-phase systems is limited by the salting-out effects of salt. The logarithm of the concentration of proteins partitioned in the salt-rich phase decreases linearly with increases in the concentration of salt in the salt-rich phase (salting-out). Therefore, the partition of a given protein in the salt-rich phase of aqueous two-phase systems can be estimated from the salting-out constant. The slope of the solubility line (salting-out constant) for a given protein is determined by the type of salt in the two-phase systems.
引用
收藏
页码：352 / 357
页数：6
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