Fast genomic μChIP-chip from 1,000 cells

被引：30
作者：
Dahl, John Arne [1 ]
Reiner, Andrew H. [1 ]
Collas, Philippe [1 ]
机构：
[1] Univ Oslo, Fac Med, Dept Biochem, Inst Basic Med Sci, N-0317 Oslo, Norway
来源：
GENOME BIOLOGY | 2009年 / 10卷 / 02期
关键词：
CHROMATIN IMMUNOPRECIPITATION ASSAY; EMBRYONIC STEM-CELLS; DNA; PLURIPOTENCY; FORMALDEHYDE; NETWORK; GENES;
D O I：
10.1186/gb-2009-10-2-r13
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Genome-wide location analysis of histone modifications and transcription factor binding relies on chromatin immunoprecipitation (ChIP) assays. These assays are, however, time-consuming and require large numbers of cells, hindering their application to the analysis of many interesting cell types. We report here a fast microChIP (mu ChIP) assay for 1,000 cells in combination with microarrays to produce genome-scale surveys of histone modifications. mu ChIP-chip reliably reproduces data obtained by large-scale assays: H3K9ac and H3K9m3 enrichment profiles are conserved and nucleosome-free regions are revealed.
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页数：11
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