Gut Microbiome and Metabolomics Profiles of Allergic and Non-Allergic Childhood Asthma

被引:19
作者
Zheng, Ping [1 ]
Zhang, Kexing [2 ]
Lv, Xifang [1 ]
Liu, Chuanhe [3 ]
Wang, Qiang [1 ]
Bai, Xuetao [1 ]
机构
[1] Chinese Ctr Dis Control & Prevent, Natl Inst Environm Hlth, China CDC Key Lab Environm & Populat Hlth, 29 Nanwei Rd, Beijing 100050, Peoples R China
[2] Xinwu Dist Ctr Dis Control & Prevent, Dept Immunizat Program, Wuxi, Jiangsu, Peoples R China
[3] Childrens Hosp, Capital Inst Pediat, Beijing, Peoples R China
来源
JOURNAL OF ASTHMA AND ALLERGY | 2022年 / 15卷
基金
中国国家自然科学基金;
关键词
gut microbiome; untargeted metabolomics; allergic childhood asthma; non-allergic childhood asthma; 16S rRNA gene sequencing; T-CELL; EARLY-LIFE; DIFFERENTIATION; RESPONSES; EXPOSURE; BACTERIA; IMMUNITY;
D O I
10.2147/JAA.S354870
中图分类号
R392 [医学免疫学];
学科分类号
100102 ;
摘要
Purpose: This study aimed to investigate the characteristics of gut bacteria and the derived metabolites among allergic asthmatic children, non-allergic asthmatic children and healthy children without asthma. Methods: Fecal samples were collected from 57 participants, including 20 healthy children, 27 allergic asthmatic children, and 10 non-allergic asthmatic children. 16S rRNA gene sequencing was conducted for analyzing gut bacterial compositions and untargeted metabolomics was used to analyze the alterations of gut microbe-derived metabolites. The associations between gut bacterial compositions and metabolites were analyzed by the method of Spearman correlation. Results: The results showed that the compositions and metabolites of gut microbiome were altered both in allergic and non-allergic asthmatics compared with healthy controls. Chaol (p = 0.025) index reflected a higher bacterial richness and Simpson (p = 0.024) index showed a lower diversity in asthma group. PERMANOVA analysis showed significant differences among the three groups based on unweighted UniFrac distance (p = 0.001). Both allergic and non-allergic asthmatics showed a higher relative abundance of Proteobacteria and a lower relative abundance of genera from Clostridia. More bacteria were altered in non-allergic asthmatics compared with allergic asthmatics. Metabolomics analysis identified that 42 metabolites were significantly associated with allergic asthma, and 58 metabolites were significantly associated with non-allergic asthma (multiple linear regression, p < 0.05). Histamine was 4 folds up-regulated only in the non-allergic asthma group. The relative abundance of Candidatus Accumulib was significantly correlated with the upregulation of histamine. The relative abundance of genera from Clostridia was significantly correlated with the downregulation of lipid and tryptophan metabolism. Conclusion: The altered gut microbes was associated with the mechanism of asthma attack through metabolites in allergic and nonallergic asthma group, respectively. The result suggested that gut microbiome had an impact on the development of both allergic and non-allergic asthma. The distinct gut microbiome and microbiome-derived metabolites in non-allergic asthma children suggested that gut microbiome might play a critical role in modulation of asthma phenotype.
引用
收藏
页码:419 / 435
页数:17
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