Aflatoxin B1 in eggs and chicken livers by dispersive liquid-liquid microextraction and HPLC

被引：58

作者：

Amirkhizi, Behzad ^{[1
,2
,3
]}

Arefhosseini, Seyed Rafie ^{[2
]}

Ansarin, Masoud ^{[1
]}

Nemati, Mahboob ^{[1
,4
]}

机构：

[1] Tabriz Univ Med Sci, Drug Appl Res Ctr, Tabriz, Iran

[2] Tabriz Univ Med Sci, Fac Nutr, Tabriz, Iran

[3] Tabriz Univ Med Sci, Fac Nutr, Students Res Comm, Tabriz, Iran

[4] Tabriz Univ Med Sci, Fac Pharm, Tabriz, Iran

来源：

FOOD ADDITIVES & CONTAMINANTS PART B-SURVEILLANCE | 2015年 / 8卷 / 04期

关键词：

aflatoxin B-1; dispersive liquid-liquid microextraction; egg; liver; HPLC; ANIMAL FEED; IRAN; MEAT; OCHRATOXIN; METABOLISM; MYCOTOXIN; PRODUCTS; RESIDUES; SAMPLES;

D O I：

10.1080/19393210.2015.1067649

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

A rapid, low-cost and simple technique has been developed for the determination of aflatoxin B-1 (AFB(1)) in eggs and livers using high-performance liquid chromatography (HPLC) with UV detection. In this study, the presence of AFB(1) was investigated in 150 eggs and 50 chicken livers from the local market of Tabriz, Iran. AFB(1) was extracted with a mixture of acetonitrile:water (80:20) and cleaned up by dispersive liquid-liquid microextraction which is a very economical, fast and sensitive method. AFB(1) was quantified by HPLC-UV without need for any complex derivatisation in samples to enhance the detection. The results showed that 72% of the liver and 58% of the egg samples were contaminated with AFB(1) ranging from 0.30 to 16.36 mu gkg (-1). limit of detection and limit of quantification for AFB(1) were 0.08 and 0.28 mu gkg (- 1), respectively. The proposed method is suitable for fast analysing of AFB(1) in egg and liver samples.

引用

页码：245 / 249

页数：5

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