Bioassay-guided isolation of triterpene from brown alga Padina boergesenii possess anti-inflammatory and anti-angiogenic potential with kinetic inhibition of β-carotene linoleate system

被引：11
作者：
Rajamani, Karthikeyan [1 ,2 ,3 ]
Balasubramanian, Thangavel [1 ]
Thirugnanasambandan, Somasundaram S. [1 ]
机构：
[1] Annamalai Univ, Ctr Adv Study Marine Biol, Parangipettai 608502, Tamil Nadu, India
[2] Annamalai Univ, Rajah Muthiah Med Coll, Annamalainagar 608002, Tamil Nadu, India
[3] Sri Ramachandra Med Coll & Res Inst, Dept Environm Hlth Engn, Madras 600116, Tamil Nadu, India
来源：
LWT-FOOD SCIENCE AND TECHNOLOGY | 2018年 / 93卷
关键词：
Padina boergesenil; Triterpene; Antioxidant; Anti-angiogenesis; Anti-inflammatory; ANTIOXIDANT ACTIVITY; ANTICOAGULANT ACTIVITY; OXIDATIVE DAMAGE; EXTRACTS; POLYPHENOLS; FRACTIONS; SARGASSUM; CAPACITY; FUCOIDAN; SEAWEED;
D O I：
10.1016/j.lwt.2018.04.010
中图分类号：
TS2 [食品工业];
学科分类号：
0832 ;
摘要：
We aimed to investigate the bioassay-guided extraction and characterization of triterpene isolated from brown alga Padina boergesenii in establishing antioxidant, anti-angiogenic and anti-inflammatory activities. The n-hexane defatted methanol crude extract (HDME) was subjected to column chromatography through combination of n-butanol:ethylacetate (BE); chloroform:ethylacetae (CE) and methanol:ethylacetate (ME) solvent system at 1:3 ratio. Five fractions (F1-F5) from each solvent system (BE, CE and ME) was collected and analyzed. Among all, the third fraction from ME (ME-F3) had potential DPPH, deoxyribose and total antioxidant activities (TAA) than CE and BE. Reducing power (RP) of ME-F3 increased with increasing concentrations of triterpene. Saturation curve of triterpene in reduction of beta-carotene bleaching at different time-points and at various concentrations have identified kinetic inhibition of free radicals. Antiangiogenic (VEGF) and anti-inflammatory (IL-1 beta) activities in A498 renal cancer cells have established the potential role of squalene. Molecular characterization by gas chromatography and mass spectrometry has identified the triterpene from ME-F3 fraction.
引用
收藏
页码：549 / 555
页数：7
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