Rapid quantification of cellular flavonoid levels using quercetin and a fluorescent diphenylboric acid 2-amino ethyl ester probe

被引：20

作者：

Lee, Ji Hae ^{[1
,2
]}

Kim, Yeojin ^{[1
,2
]}

Hoang, Minh Hien ^{[1
,2
]}

Jun, Hee-jin ^{[1
,2
]}

Lee, Sung-Joon ^{[1
,2
]}

机构：

[1] Korea Univ, Grad Sch Life Sci & Biotechnol, Dept Biotechnol, Seoul 136713, South Korea

[2] Korea Univ, Div Food Biosci & Technol, Coll Life Sci & Biotechnol, Seoul 136713, South Korea

来源：

FOOD SCIENCE AND BIOTECHNOLOGY | 2014年 / 23卷 / 01期

基金：

新加坡国家研究基金会;

关键词：

flavonoids; spectrofluorometry; fluorescence activated cell sorter (FACS); diphenylboric acid 2-aminoethyl ether (DPBA); quercetin; DIETARY FLAVONOIDS; BIOAVAILABILITY;

D O I：

10.1007/s10068-014-0010-y

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

Flavonoids are abundant in fruits and vegetables and have various biological activities. A rapid, sensitive method for assessing cellular quercetin levels is necessary because cellular levels are often low. A new method for measuring cellular flavonoid levels is proposed using diphenylboric acid 2-aminoethyl ester (DPBA), a fluorescent probe, and quercetin, a representative flavonoid. This spectrofluorometry-based assay is simple and allows rapid quantification of the cellular quercetin content with high sensitivity. CHO-K1 cells were incubated with quercetin for 5 min, and then the cellular quercetin concentration was assayed using DPBA spectrofluorometry. Results, compared with high-performance liquid chromatography (HPLC), thin layer chromatography (TLC), and conventional spectrophotometry measurements, were highly correlated with HPLC data (R > 0.99). The method is more sensitive than TLC and conventional spectrophotometry. Spectrofluorometric measurement using DPBA is a simple, rapid, and sensitive method for assessing cellular quercetin levels.

引用

页码：75 / 79

页数：5

共 18 条

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