Melittin radiosensitizes esophageal squamous cell carcinoma with induction of apoptosis in vitro and in vivo

被引：26

作者：

Zhu, Hongcheng ^{[1
]}

Yang, Xi ^{[1
]}

Liu, Jia ^{[1
]}

Ge, Yangyang ^{[2
]}

Qin, Qin ^{[1
]}

Lu, Jing ^{[1
]}

Zhan, Liangliang ^{[1
]}

Liu, Zheming ^{[1
]}

Zhang, Hao ^{[1
]}

Chen, Xiaochen ^{[1
]}

Zhang, Chi ^{[1
]}

Xu, Liping ^{[1
]}

Cheng, Hongyan ^{[3
]}

Sun, Xinchen ^{[1
]}

机构：

[1] Nanjing Med Univ, Affiliated Hosp 1, Dept Radiat Oncol, Nanjing 210029, Jiangsu, Peoples R China

[2] Nantong Tumor Hosp, Dept Radiat Oncol, Nantong 226361, Peoples R China

[3] Nanjing Med Univ, Affiliated Hosp 1, Dept Gen Internal Med, Nanjing 210029, Jiangsu, Peoples R China

来源：

TUMOR BIOLOGY | 2014年 / 35卷 / 09期

关键词：

Melittin; Esophageal squamous cell carcinoma; Radiosensitivity; Apoptosis; CANCER CELLS; BEE VENOM; INHIBITION; ACTIVATION; EXPRESSION; PEPTIDES; PROTEINS; GROWTH; DEATH; CHINA;

D O I：

10.1007/s13277-014-2146-z

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Currently, unresectable esophageal squamous cell carcinoma (ESCC) is primarily treated by chemoradiotherapy. However, the outcome has not improved significantly because of radioresistance of cancer cells. This study aimed to determine the radiosensitizing effect of melittin, a novel component of bee venom, in ESCC. ESCC cell lines were irradiated with or without melittin. Cell proliferation was detected by Cell Counting Kit 8 assay. Radiosensitization was evaluated by clonogenic survival assay. Cell apoptosis was detected by flow cytometry. Results show that melittin potently sensitized ESCC cells to radiation with a sensitization enhancement ratio of 1.15-1.42. Radiosensitization was accompanied with enhanced apoptosis and regulated by apoptosis proteins. The results were confirmed by in vivo studies on tumor-bearing xenografts. In summary, these results provide support that melittin may be a potentially promising radiosensitizer in ESCC radiation therapy.

引用

页码：8699 / 8705

页数：7

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