Exploring the role of polymer structure on intracellular nucleic acid delivery via polymeric nanoparticles

被引：64
作者：
Bishop, Corey J. [1 ]
Kozielski, Kristen L. [1 ]
Green, Jordan J. [1 ,2 ,3 ,4 ]
机构：
[1] Johns Hopkins Univ, Sch Med, Translat Tissue Engn Ctr, Inst Nanobiotechnol,Dept Biomed Engn, Baltimore, MD 21218 USA
[2] Johns Hopkins Univ, Sch Med, Dept Neurosurg, Dept Oncol, Baltimore, MD USA
[3] Johns Hopkins Univ, Sch Med, Dept Ophthalmol, Baltimore, MD USA
[4] Johns Hopkins Univ, Dept Mat Sci & Engn, Baltimore, MD 21218 USA
来源：
JOURNAL OF CONTROLLED RELEASE | 2015年 / 219卷
关键词：
GENE DELIVERY; IN-VITRO; SIRNA DELIVERY; DNA DELIVERY; PLASMID DNA; CATIONIC POLYMER; CELLULAR UPTAKE; POLY(BETA-AMINO ESTER); MOLECULAR-WEIGHT; TRANSFECTION EFFICIENCY;
D O I：
10.1016/j.jconrel.2015.09.046
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
Intracellular nucleic acid delivery has the potential to treat many genetically-based diseases, however, gene delivery safety and efficacy remains a challenging obstacle. One promising approach is the use of polymers to form polymeric nanoparticles with nucleic acids that have led to exciting advances in non-viral gene delivery. Understanding the successes and failures of gene delivery polymers and structures is the key to engineering optimal polymers for gene delivery in the future. This article discusses the polymer structural features that enable effective intracellular delivery of DNA and RNA, including protection of nucleic acid cargo, cellular uptake, endosomal escape, vector unpacking, and delivery to the intracellular site of activity. The chemical properties that aid in each step of intracellular nucleic acid delivery are described and specific structures of note are highlighted. Understanding the chemical design parameters of polymeric nucleic acid delivery nanoparticles is important to achieving the goal of safe and effective non-viral genetic nanomedicine. (C) 2015 Elsevier B.V. All rights reserved.
引用
收藏
页码：488 / 499
页数：12
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