Protective effects of a wheat germ peptide (RVF) against H2O2-induced oxidative stress in human neuroblastoma cells

被引：24

作者：

Cheng, Yunhui ^{[1
]}

Zhang, Li ^{[2
]}

Sun, Wei ^{[1
]}

Tang, Jiqing ^{[1
]}

Lv, Zhaoliang ^{[1
]}

Xu, Zhou ^{[1
]}

Yu, Huixin ^{[2
]}

机构：

[1] Changsha Univ Sci & Technol, Sch Chem & Biol Engn, Changsha 410114, Hunan, Peoples R China

[2] Jiangsu Inst Nucl Med, Jiangsu Key Lab Mol Nucl Med, Minist Hlth, Key Lab Nucl Med, Wuxi 214063, Jiangsu, Peoples R China

来源：

BIOTECHNOLOGY LETTERS | 2014年 / 36卷 / 08期

基金：

中国国家自然科学基金;

关键词：

Arg-Val-Phe; Apoptosis; Human neuroblastoma cells; Oxidative stress; Peptide; Poly(ADP-ribose) polymerase; Wheat germ peptide (RVF); APOPTOSIS; PROTEIN;

D O I：

10.1007/s10529-014-1521-6

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

RVF (Arg-Val-Phe), a peptide derived from wheat germ, shows antioxidant properties. Here, the neuroprotective efficacies of RVF were investigated in human neuroblastoma cells (SH-SY5Y) that were pretreated with RVF (150-250 mu M, 4 h) and exposed to H2O2 (200 mu M). RVF increased viable cell numbers by 37 % and reduced the release of lactate dehydrogenase. Pretreatment with RVF also inhibited H2O2-induced accumulation of reactive oxygen species and maintained the mitochondrial transmembrane potential as well as preventing intracellular Ca2+ dysregulation during H2O2 exposure. Furthermore, pretreatment with RVF increased the Bcl-2/Bax ratio and blocked cleavage poly(ADP-ribose) polymerase by inhibiting caspase-3 activation, thus decreasing apoptosis.

引用

页码：1615 / 1622

页数：8

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