Co-inoculation of cellulolytic rumen bacteria with methanogenic sludge to enhance methanogenesis of rice straw

Ruminal microbiota co-inoculated with methanogenic sludge were applied in a 2 L anaerobic system. The experiment lasted 48 days in three stages with different substrate loading rates of 3 g d(-1), 7 g d(-1) and 14 g d(-1). The fermentation performance and microbial characteristics were assessed. The mean methane yields of different stages increased 1.79-, 2.07-and 2.26-fold compared with control samples, while the methane content of biogas varied from 32 to 44%, significantly different (P < 0.05) from the control reactor. There was no corresponding accumulation of volatile fatty acids (VFAs) and pH values varied between 6.8 and 7.68 during the whole anaerobic digestion, indicating system stability. The cellulolytic activities improved significantly (P < 0.05) compared with control samples. Co-inoculation not only added methanogens but also increased the proportion of bacteria containing GH 5 genes even though the bacterial composition changed by PCR-DGGE phylogenetic analysis. The proportion of cellulolytic genera of Clostridium and Ruminococcus increased, whereas Bacteroides, Fibrobacter and Acetivibrio disappeared due to their inability to acclimatize to in vitro lignocellulosic conditions. The analysis of GH and 16S rRNA genes also provided a better description of cellulolytic function and phylogenetic profile. Mutual cooperation formed between Methanobrevibacter and Ruminococcus was achieved for high cellulolytic activity and methanogenic efficiency in co-inoculated system. Moreover, this hypothesis was further strengthened by observations on morphological characteristics of cellulolytic consortia. (C) 2017 Elsevier Ltd. All rights reserved.