MicroRNA-144 inhibits hepatocellular carcinoma cell proliferation, invasion and migration by targeting ZFX

被引:36
作者
Bao, Hongbin [1 ]
Li, Xinguo [1 ]
Li, Hengli [1 ]
Xing, Hongli [1 ]
Xu, Binghui [1 ]
Zhang, Xianfeng [1 ]
Liu, Zhaoming [1 ]
机构
[1] Harrison Int Peace Hosp, Dept Hepatobiliary Surg, Hengshui City, Hebei Province, Peoples R China
关键词
Cell proliferation; hepatocellular carcinoma; invasion; migration; miR-144; ZFX; DOWN-REGULATION; CANCER CELLS; EXPRESSION; MIR-144; MTOR;
D O I
10.1007/s12038-016-9662-5
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
MicroRNA 144 (miR-144), a small non-coding RNA, is frequently dysregulated in human several tumour progression, but its role and the underlying mechanisms in hepatocellular carcinoma (HCC) is poorly investigated. In the present study, the expression of miR-144 was firstly analysed in datasets derived from GSE21362 and TCGA, and then detected in HCC tissues and cell lines by quantitative RT-PCR (qRT-PCR) analysis. MiR-144 was shown to be significantly down-regulated in HCC tissues and cell lines. Subsequently, overexpression of miR-144 was transfected into HCC cell lines so as to investigate its biological function, including MTT, colony formation, and transwell assays. Gain of function assay revealed miR-144 remarkably inhibited cell proliferation, migration and invasion. In addition, bioinformatical analysis and luciferase reporter assay identified ZFX as a novel target of miR-144 in HCC cells, as confirmed by qRT-PCR and Western blot. Furthermore, ZFX was found to be significantly up-regulated using Oncomine database analysis. Loss of function assay further indicated knockdown of ZFX had similar effects of miR-144-mediated HCC cell proliferation and invasion. Therefore, miR-144 has been demonstrated to act as a tumour suppressor in HCC cell growth and motility by directly targeting ZFX, which implicates its potential applications in the development of HCC treatment.
引用
收藏
页码:103 / 111
页数:9
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