Secretome From Mesenchymal Stem Cells Induces Angiogenesis Via Cyr61

被引：114

作者：

Estrada, Rosendo ^{[1
]}

Li, Na ^{[1
]}

Sarojini, Harshini ^{[1
]}

An, Jin ^{[1
]}

Lee, Menq-Jer ^{[1
,2
]}

Wang, Eugenia ^{[1
,3
]}

机构：

[1] Univ Louisville, Sch Med, Gheens Ctr Aging, Louisville, KY 40202 USA

[2] Univ Louisville, Sch Med, Dept Microbiol & Immunol, Louisville, KY 40202 USA

[3] Univ Louisville, Sch Med, Dept Biochem & Mol Biol, Louisville, KY 40202 USA

来源：

JOURNAL OF CELLULAR PHYSIOLOGY | 2009年 / 219卷 / 03期

关键词：

IMMEDIATE-EARLY GENE; GROWTH-FACTOR; STROMAL CELLS; BONE-MARROW; ENDOTHELIAL-CELLS; CCN FAMILY; ADHESION; FIBROBLASTS; PRODUCT; DIFFERENTIATION;

D O I：

10.1002/jcp.21701

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

It is well known that bone marrow-derived mesenchymal stem cells (MSCs) are involved in wound healing and regeneration responses. In this study, we globally profiled the proteome of MSCs to investigate critical factor(s) that may promote wound healing. Cysteine-rich protein 61 (Cyr61) was found to be abundantly present in MSCs. The presence of Cyr61 was confirmed by immunofluorescence staining and immunoblot analysis. Moreover, we showed that Cyr61 is present in the culture medium (secretome) of MSCs. The secretome of MSCs stimulates angiogenic response in vitro, and neovascularization in vivo. Depletion of Cyr61 completely abrogates the angiogenic-inducing capability of the MSC secretome. Importantly, addition of recombinant Cyr61 polypeptides restores the angiogenic activity of Cyr61-depleted secretome. Collectively, these data demonstrate that Cyr61 polypeptide in MSC secretome contributes to the angiogenesis-promoting activity, a key event needed for regeneration and repair of injured tissues.

引用

页码：563 / 571

页数：9

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