Mini-chromosomes derived from the human Y chromosome by telomere directed chromosome breakage

被引：124

作者：

Heller, R ^{[1
]}

Brown, KE ^{[1
]}

Burgtorf, C ^{[1
]}

Brown, WRA ^{[1
]}

机构：

[1] UNIV OXFORD,DEPT BIOCHEM,CANC RES CAMPAIGN,CHROMOSOME MOLEC BIOL GRP,OXFORD OX1 3QU,ENGLAND

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1996年 / 93卷 / 14期

关键词：

centromere; artificial chromosome;

D O I：

10.1073/pnas.93.14.7125

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

We have used telomeric DNA to break two acrocentric derivatives of the human Y chromosome into mini-chromosomes that are small enough to be size-fractionated by pulsed-field gel electrophoresis. One of the mini-chromosomes is about 7 Mb in size and sequence-tagged site analysis of this molecule suggests that it corresponds to a simple truncation of the short arm of the Y chromosome. Five of the mini-chromosomes are derived from the long arm, are all rearranged by more than a simple truncation, and range in size from 4.0 Mb to 9 Mb. We have studied the mitotic stabilities of these mini-chromosomes and shown that they are stably maintained by cells proliferating in culture for about 100 cell divisions.

引用

页码：7125 / 7130

页数：6

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