High-quality draft genome sequence of nematocidal Bacillus thuringiensis Sbt003

被引：3
|
作者：
Liu, Yingying [1 ]
Ye, Weixing [1 ]
Zheng, Jinshui [1 ]
Fang, Lei [1 ]
Peng, Donghai [1 ]
Ruan, Lifang [1 ]
Sun, Ming [1 ]
机构：
[1] Huazhong Agr Univ, Coll Life Sci & Technol, State Key Lab Agr Microbiol, Wuhan, Peoples R China
来源：
STANDARDS IN GENOMIC SCIENCES | 2014年 / 9卷 / 03期
基金：
中国国家自然科学基金;
关键词：
The Next-Generation sequencing; parasporal crystal protein; Bacillus thuringiensis; CEREUS; BACTERIA; ALIGNMENT; ANTHRACIS; BIOLOGY; GENES; TOOL;
D O I：
10.4056/sigs.4738557
中图分类号：
Q3 [遗传学];
学科分类号：
071007 ; 090102 ;
摘要：
Bacillus thuringiensis represents one of the six species of "Bacillus cereus group" in the genus Bacillus within the family Bacillaceae. Strain Sbt003 was isolated from soil and identified as B. thuringiensis. It harbors at least seven plasmids and produces three shapes of parasporal crystals including oval, bipyramidal and rice. SDS-PAGE analysis of spore-crystal suspension of this strain reveals six major protein bands, which implies the presence of multiple parasporal crystal genes. Bioassay of this strain reveals that it shows specific activity against nematodes and human cancer cells. In this study, we report the whole genomic shotgun sequences of Sbt003. The high-quality draft of the genome is 6,175,670 bp long (including chromosome and plasmids) with 6,372 protein-coding and 80 RNA genes.
引用
收藏
页码：624 / 631
页数：8
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