A lysosome-targetable near infrared fluorescent probe for glutathione sensing and live-cell imaging

被引:38
作者
Zheng, Ziming [1 ]
Huyan, Yuchen [1 ]
Li, Hongjuan [1 ]
Sun, Shiguo [1 ]
Xu, Yongqian [1 ]
机构
[1] Northwest A&F Univ, Coll Chem & Pharm, Shaanxi Key Lab Nat Prod & Chem Biol, Yangling 712100, Shaanxi, Peoples R China
基金
中国国家自然科学基金;
关键词
Squaraine dye; Enhanced self-quenching; GSH; Cell imaging; Lysosomal organelle; SELECTIVE DETECTION; GOLD NANOPARTICLES; THIOLS; ASSAY; HOMOCYSTEINE; ACID;
D O I
10.1016/j.snb.2019.127065
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
A novel near-infrared self-quenching dye SQSS consisting of two SQ fluorophores and a cystamine linker was constructed for the selective detection of GSH. Owing to the enhanced aggregation-caused quenching (ACQ) and FRET-mediated self-quenching effects, the background fluorescence intensity of SQSS is very weak, providing relatively high signal-to-noise ratio. The disulfide linker of SQSS can be selectively cleaved by glutathione (GSH) rather than other biothiols to produce two SQ derivatives through cooperative intermolecular hydrogen bonds and specific electrostatic interactions. The exclusive interaction of GSH with SQSS interrupt the FRET self-quenching effects between two SQ fluorophores, thereby generating a significant turn on spectral response for detection of GSH in near infrared region. Cell imaging experiments showed that SQSS can monitor endogenous and exogenous GSH in tumor or normal cells. More importantly, colocalization experiments indicated that SQSS mainly located in the lysosome, an organelle tightly associated with GSH functions. With the excellent membrane permeability and lysosome-specificity, it is convinced that SQSS will be a powerful tool in the future research on the function of GSH in lysosomes.
引用
收藏
页数:7
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共 43 条
[1]   Enzymatic reduction of disulfide bonds in lysosomes: Characterization of a Gamma-interferon-inducible lysosomal thiol reductase (GILT) [J].
Arunachalam, B ;
Phan, UT ;
Geuze, HJ ;
Cresswell, P .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2000, 97 (02) :745-750
[2]   The in vivo sparing of methionine by cysteine in sulfur amino acid requirements in animal models and adult humans [J].
Ball, Ronald O. ;
Courtney-Martin, Glenda ;
Pencharz, Paul B. .
JOURNAL OF NUTRITION, 2006, 136 (06) :1682S-1693S
[3]   Fluorescent and colorimetric probes for detection of thiols [J].
Chen, Xiaoqiang ;
Zhou, Ying ;
Peng, Xiaojun ;
Yoon, Juyoung .
CHEMICAL SOCIETY REVIEWS, 2010, 39 (06) :2120-2135
[4]   Sensitive and selective detection of glutathione based on resonance light scattering using sensitive gold nanoparticles as colorimetric probes [J].
Chen, Zhanguang ;
Wang, Zhen ;
Chen, Junhui ;
Wang, Shaobin ;
Huang, Xiaopeng .
ANALYST, 2012, 137 (13) :3132-3137
[5]   In Vivo Imaging of Endogenously Produced HClO in Zebrafish and Mice Using a Bright, Photostable Ratiometric Fluorescent Probe [J].
Duan, Chong ;
Won, Miae ;
Verwilst, Peter ;
Xu, Junchao ;
Kim, Hyeong Seok ;
Zeng, Lintao ;
Kim, Jong Seung .
ANALYTICAL CHEMISTRY, 2019, 91 (06) :4172-4178
[6]   A distinctive near-infrared fluorescence turn-on probe for rapid, sensitive and chromogenic detection of sulfite in food [J].
Duan, Chong ;
Zhang, Jun-Feng ;
Hu, Yubo ;
Zeng, Lintao ;
Su, Dongdong ;
Bao, Guang-Ming .
DYES AND PIGMENTS, 2019, 162 :459-465
[7]   A Two-Photon Fluorescent Probe for Lysosomal Thiols in Live Cells and Tissues [J].
Fan, Jiangli ;
Han, Zhichao ;
Kang, Yao ;
Peng, Xiaojun .
SCIENTIFIC REPORTS, 2016, 6
[8]   A BSA-squaraine hybrid system for selectively detecting Ag+ in absolute PBS and sequential construction of logic functions [J].
Fan, Xiaopeng ;
Zhang, Dan ;
Li, Hongjuan ;
Sun, Shiguo ;
Xu, Yongqian .
SENSORS AND ACTUATORS B-CHEMICAL, 2017, 245 :290-296
[9]   Analysis of GSH and GSSG after derivatization with N-ethylmaleimide [J].
Giustarini, Daniela ;
Dalle-Donne, Isabella ;
Milzani, Aldo ;
Fanti, Paolo ;
Rossi, Ranieri .
NATURE PROTOCOLS, 2013, 8 (09) :1660-1669
[10]   A disulfide bound-molecular beacon as a fluorescent probe for the detection of reduced glutathione and its application in cells [J].
Guo, Yingshu ;
Wang, Hao ;
Sun, Yuanshun ;
Qu, Bin .
CHEMICAL COMMUNICATIONS, 2012, 48 (26) :3221-3223