Baculovirus expression: old dog, new tricks

被引：17
作者：
Berger, Imre [1 ,2 ,3 ]
Poterszman, Arnaud [4 ,5 ,6 ,7 ]
机构：
[1] Univ Grenoble Alpes, CNRS, EMBL, Grenoble Outstn,UMR 5233, Grenoble, France
[2] Univ Grenoble Alpes, CNRS, EMBL, UVHCI,UMR 5233, Grenoble, France
[3] Univ Bristol, Sch Biochem, Clifton, England
[4] CBI, IGBMC, Dept Integrated Struct Biol, Illkirch Graffenstaden, France
[5] CNRS, UMR 7104, Illkirch Graffenstaden, France
[6] INSERM, U964, Illkirch Graffenstaden, France
[7] Univ Strasbourg, Strasbourg, France
来源：
BIOENGINEERED | 2015年 / 6卷 / 06期
关键词：
automation; baculovirus; DNA recombineering; insect cell culture; multiprotein complex; protein modification; recombinant expression; structural biology; synthetic biology; INSECT CELLS; PROTEIN EXPRESSION; STRUCTURAL BIOLOGY; RECOMBINANT BACULOVIRUSES; MULTIPROTEIN COMPLEXES; THROUGHPUT PRODUCTION; MULTIGENE EXPRESSION; ESCHERICHIA-COLI; VECTOR SYSTEMS; DNA;
D O I：
10.1080/21655979.2015.1104433
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Since its inception more than 30years ago, the baculovirus expression vector system (BEVS) has been used prolifically to produce heterologous proteins for research and development. In the cell, a cornerstone of biological activity are multiprotein complexes, catalyzing essential functions. BEVS has been uniquely successful to unlock such complex assemblies for high-resolution structural and functional analysis. Synthetic biology approaches have been implemented to optimize multigene assembly methods, accelerating upstream processes. Specialized baculoviral genomes are being created with functions tailored to enhance production of particular target protein classes. Here we comment on current and emerging developments in the field and their potential to accelerate protein complex research.
引用
收藏
页码：316 / 322
页数：7
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