HJURP Is a Cell-Cycle-Dependent Maintenance and Deposition Factor of CENP-A at Centromeres

被引：505

作者：

Dunleavy, Elaine M. ^{[1
]}

Roche, Daniele ^{[1
]}

Tagami, Hideaki ^{[2
]}

Lacoste, Nicolas ^{[1
]}

Ray-Gallet, Dominique ^{[1
]}

Nakamura, Yusuke ^{[3
]}

Daigo, Yataro ^{[3
]}

Nakatani, Yoshihiro ^{[2
]}

Almouzni-Pettinotti, Genevieve ^{[1
]}

机构：

[1] Inst Curie, CNRS, Lab Nucl Dynam & Genome Plast, UMR218, F-75248 Paris 05, France

[2] Nagoya City Univ, Grad Sch Nat Sci, Mizuho Ku, Aichi 4678501, Japan

[3] Univ Tokyo, Inst Med Sci, Ctr Human Genome, Mol Med Lab, Tokyo 1130033, Japan

来源：

CELL | 2009年 / 137卷 / 03期

关键词：

HISTONE H3 VARIANT; FISSION YEAST SCM3; BUDDING YEAST; CHROMATIN; PROTEIN; KINETOCHORE; COMPLEX; NUCLEOSOME; CHAPERONE; DNA;

D O I：

10.1016/j.cell.2009.02.040

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The histone H3 variant CenH3, called CENP-A in humans, is central in centromeric chromatin to ensure proper chromosome segregation. In the absence of an underlying DNA sequence, it is still unclear how CENP-A deposition at centromeres is determined. Here, we purified non-nucleosomal CENP-A complexes to identify direct CENP-A partners involved in such a mechanism and identified HJURP. HJURP was not detected in H3.1- or H3.3-containing complexes, indicating its specificity for CENP- A. HJURP centromeric localization is cell cycle regulated, and its transient appearance at the centromere coincides precisely with the proposed time window for new CENP- A deposition. Furthermore, HJURP downregulation leads to a major reduction in CENP-A at centromeres and impairs deposition of newly synthesized CENP-A, causing mitotic defects. We conclude that HJURP is a key factor for CENP-A deposition and maintenance at centromeres.

引用

页码：485 / 497

页数：13

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