Structure and Immune Recognition of the HIV Glycan Shield

被引:98
作者
Crispin, Max [1 ,2 ]
Ward, Andrew B. [3 ,4 ]
Wilson, Ian A. [3 ,4 ,5 ]
机构
[1] Univ Southampton, Ctr Biol Sci, Southampton SO17 1BJ, Hants, England
[2] Univ Southampton, Inst Life Sci, Southampton SO17 1BJ, Hants, England
[3] Scripps Res Inst, Int AIDS Vaccine Initiat Neutralizing Antibody Ct, Ctr HIV AIDS Vaccine Immunol & Immunogen Discover, Dept Integrat Struct & Computat Biol, La Jolla, CA 92037 USA
[4] Scripps Res Inst, Collaborat AIDS Vaccine Discovery, La Jolla, CA 92037 USA
[5] Scripps Res Inst, Skaggs Inst Chem Biol, La Jolla, CA 92037 USA
来源
ANNUAL REVIEW OF BIOPHYSICS, VOL 47 | 2018年 / 47卷
关键词
viral glycoprotein; broadly neutralizing antibody; glycosylation; glycan composition; EM structure; X-ray structure; BROADLY NEUTRALIZING ANTIBODIES; ENVELOPE GLYCOPROTEIN TRIMERS; CRYO-EM STRUCTURE; SIMIAN/HUMAN IMMUNODEFICIENCY VIRUS; CD4; BINDING-SITE; INFLUENZA-VIRUS; GLYCOSYLATION PROFILES; MONOCLONAL-ANTIBODIES; DEPENDENT EPITOPE; CRYSTAL-STRUCTURE;
D O I
10.1146/annurev-biophys-060414-034156
中图分类号
Q6 [生物物理学];
学科分类号
071011 ;
摘要
Vaccine design efforts against the human immunodeficiency virus (HIV) have been greatly stimulated by the observation that many infected patients eventually develop highly potent broadly neutralizing antibodies (bnAbs). Importantly, these bnAbs have evolved to recognize not only the two protein components of the viral envelope protein (Env) but also the numerous glycans that form a protective barrier on the Env protein. Because Env is heavily glycosylated compared to host glycoproteins, the glycans have become targets for the antibody response. Therefore, considerable efforts have been made in developing and validating biophysical methods to elucidate the complex structure of the Env-spike glycoprotein, with its combination of glycan and protein epitopes. We illustrate here how the application of robust biophysical methods has transformed our understanding of the structure and function of the HIV Env spike and stimulated innovation in vaccine design strategies that takes into account the essential glycan components.
引用
收藏
页码:499 / 523
页数:25
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