Biomimetic PCL-gelatin based nanofibers loaded with ciprofloxacin hydrochloride and quercetin: A potential antibacterial and anti-oxidant dressing material for accelerated healing of a full thickness wound

被引:142
作者
Ajma, Gufran [1 ]
Bonde, Gunjan Vasant [1 ]
Mittal, Pooja [1 ]
Khan, Gayasuddin [1 ]
Pandey, Vivek Kumar [2 ]
Bakade, Bharati, V [3 ]
Mishra, Brahmeshwar [1 ]
机构
[1] Indian Inst Technol BHU, Dept Pharmaceut Engn & Technol, Varanasi, Uttar Pradesh, India
[2] Indian Inst Technol BHU, Dept Chem Engn & Technol, Varanasi, Uttar Pradesh, India
[3] Pataldhamal Wadhwani Coll Pharm, Yavatmal, Maharashtra, India
关键词
Ciprofloxacin hydrochloride; Quercetin; PCL; Gelatin; Electrospun nanofiber; Wound Healing; CONTROLLED-RELEASE; ELECTROSPUN; PCL/GELATIN; SCAFFOLDS; MEMBRANE; COLLAGEN; MATS;
D O I
10.1016/j.ijpharm.2019.118480
中图分类号
R9 [药学];
学科分类号
1007 ;
摘要
An open wound is highly susceptible to microbial infection leading to elevated level of inflammatory response. For prompt healing, a wound requires a biomimetic dressing material with ideal hydrophilicity and tensile strength, possessing antimicrobial and antioxidant property. Although PCL-based nanofibers have sufficient tensile strength and biocompatibility, it lacks in terms of optimum hydrophilicity and biodegradation. Therefore, we fabricated a PCL-gelatin based electrospun nanofibers, enriched with quercetin and ciprofloxacin hydrochloride (CH). The average diameter of developed nanofibers was 725.943 +/- 201.965 nm, and devoid of chemical interaction between two drugs and polymers. CH and quercetin exhibited biphasic in-vitro release in phosphate buffer (pH 7.4). The in-vitro antibacterial and antioxidant property of scaffolds were evaluated by film-diffusion against Staphylococcus aureus and DPPH assay, respectively. The addition of gelatin along with CH and quercetin enhanced the hydrophilicity (contact angle = 48.8 +/- 2.95 degrees) and biodegradation rate of the nanofibers. In-vitro biocompatibility of scaffold was examined by hemocompatibility and fibroblast viability using MTT assay. The results confirm the direct application of scaffold in the wounded area. Further, complete closure of full-thickness wound within 16 days, and regulation of hydroxyproline, SOD and catalase level in granulation tissues following treatment with scaffold, confirmed its application for accelerated wound healing.
引用
收藏
页数:12
相关论文
共 40 条
[1]  
AEBI H, 1984, METHOD ENZYMOL, V105, P121
[2]   Ciprofloxacin HCl and quercetin functionalized electrospun nanofiber membrane: fabrication and its evaluation in full thickness wound healing [J].
Ajmal, Gufran ;
Bonde, Gunjan Vasant ;
Thokala, Sathish ;
Mittal, Pooja ;
Khan, Gayasuddin ;
Singh, Juhi ;
Pandey, Vivek Kumar ;
Mishra, Brahmeshwar .
ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY, 2019, 47 (01) :228-240
[3]   Biocomposite nanofiber matrices to support ECM remodeling by human dermal progenitors and enhanced wound closure [J].
Anjum, Fraz ;
Agabalyan, Natacha A. ;
Sparks, Holly D. ;
Rosin, Nicole L. ;
Kallos, Michael S. ;
Biernaskie, Jeff .
SCIENTIFIC REPORTS, 2017, 7
[4]  
[Anonymous], 2016, INFLAMMATION IMMUNIT
[5]   Electrodeposition of quercetin on the electrospun zinc oxide nanofibers and its application as a sensing platform for uric acid [J].
Arvand, Majid ;
Tajyani, Sepideh ;
Ghodsi, Navid .
MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS, 2015, 46 :325-332
[6]   SUPEROXIDE DISMUTASE - IMPROVED ASSAYS AND AN ASSAY APPLICABLE TO ACRYLAMIDE GELS [J].
BEAUCHAM.C ;
FRIDOVIC.I .
ANALYTICAL BIOCHEMISTRY, 1971, 44 (01) :276-&
[7]   LATE DEGRADATION TISSUE-RESPONSE TO POLY(L-LACTIDE) BONE PLATES AND SCREWS [J].
BERGSMA, JE ;
DEBRUIJN, WC ;
ROZEMA, FR ;
BOS, RRM ;
BOERING, G .
BIOMATERIALS, 1995, 16 (01) :25-31
[8]   Evaluation of electrospun PCL/gelatin nanofibrous scaffold for wound healing and layered dermal reconstitution [J].
Chong, E. J. ;
Phan, T. T. ;
Lim, I. J. ;
Zhang, Y. Z. ;
Bay, B. H. ;
Ramakrishna, S. ;
Lim, C. T. .
ACTA BIOMATERIALIA, 2007, 3 (03) :321-330
[9]  
da Silva EC, 2016, GLOBAL ADVANCES IN SELENIUM RESEARCH FROM THEORY TO APPLICATION, P133
[10]   Biodegradation of bicomponent PCL/gelatin and PCL/collagen nanofibers electrospun from alternative solvent system [J].
Dulnik, J. ;
Denis, P. ;
Sajkiewicz, P. ;
Kolbuk, D. ;
Choinska, E. .
POLYMER DEGRADATION AND STABILITY, 2016, 130 :10-21