Structural analysis of O-glycans of mucin from jellyfish (Aurelia aurita) containing 2-aminoethylphosphonate

被引：19

作者：

Urai, Makoto ^{[1
]}

Nakamura, Takemichi ^{[2
]}

Uzawa, Jun ^{[1
]}

Baba, Takayuki ^{[1
]}

Taniguchi, Kayoko ^{[1
]}

Seki, Hiroko ^{[3
]}

Ushida, Kiminori ^{[1
]}

机构：

[1] RIKEN, Ecosoft Mat Res Unit, Adv Sci Inst, Wako, Saitama 3510198, Japan

[2] RIKEN, Mol Characterizat Team, Adv Sci Inst, Wako, Saitama 3510198, Japan

[3] Chiba Univ, Ctr Chem Anal, Inage Ku, Chiba 2638522, Japan

来源：

CARBOHYDRATE RESEARCH | 2009年 / 344卷 / 16期

关键词：

Jellyfish; Glycoprotein; Mucin; Glycan; Structure; 2-Aminoethylphosphonate; PERFORMANCE LIQUID-CHROMATOGRAPHY; MASS-SPECTROMETRY; APLYSIA-KURODAI; NERVOUS-SYSTEM; GLYCOPROTEIN; ACID; OLIGOSACCHARIDES; QNIUMUCIN; LUBRICIN;

D O I：

10.1016/j.carres.2009.08.001

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The structure of O-glycan in qniumucin (Q-mucin), which is a novel mucin extracted from jellyfish, was analyzed by a combination of NMR and ESI-MS/MS. A previously unidentified monosaccharide involved in the glycan chains was determined to be N-acetylgalactosamine (GalNAc) substituted by 2-aminoethylphosphonate (AEP) at the C-6. The O-glycans in Q-mucin from Aurelia aurita were proved to be mainly composed of three monosaccharides: GalNAc, AEP-(O -> 6)-GalNAc, and P-6-GalNAc. To the best of our knowledge, this is the first example of an O-glycan structure of glycoproteins containing AEP. This exceptionally simple structure of Q-mucin and its potential use in material science and technology are revealed. (C) 2009 Elsevier Ltd. All rights reserved.

引用

页码：2182 / 2187

页数：6

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