Quantitative analysis of adsorption and desorption behavior of individual cellulase components during the hydrolysis of lignocellulosic biomass with the addition of lysozyme

被引：10

作者：

Toyosawa, Yoshiko ^{[1
]}

Ikeo, Makoto ^{[1
]}

Taneda, Daisuke ^{[1
]}

Okino, Shohei ^{[1
]}

机构：

[1] JGC Corp, 2205 Narita Cho, Oarai, Ibaraki 3111313, Japan

来源：

BIORESOURCE TECHNOLOGY | 2017年 / 234卷

关键词：

Lignocellulosic bioethanol; Lysozyme; Non-specific adsorption; Enzyme adsorption/desorption; Sugarcane bagasse; ENZYMATIC-HYDROLYSIS; SUGARCANE BAGASSE; LIGNIN; ENZYMES; SACCHARIFICATION; COST;

D O I：

10.1016/j.biortech.2017.02.132

中图分类号：

S2 [农业工程];

学科分类号：

0828 ;

摘要：

The effect of non-catalytic protein addition on the adsorption/desorption behavior of individual cellulase components on/from substrates during the hydrolysis of microcrystalline cellulose and steam exploded sugarcane bagasse (SEB) were investigated. The addition of non-catalytic protein enhanced the enzymatic hydrolysis of SEB, but did not enhance the hydrolysis of microcrystalline cellulose. During the hydrolysis of SEB, adsorption of beta-glucosidase (BGL) was prevented in the presence of non-catalytic protein. Cellobiohydrolase I (CBH I) and endoglucanase I (EG I) desorbed from the substrate after temporary adsorption in the presence of non-catalytic protein during SEB hydrolysis. This suggested that reduction of the non-specific adsorption of cellulase components, CBH I, EG I, and BGL, on lignin in SEB led to the improving of enzymatic hydrolysis. (C) 2017 Elsevier Ltd. All rights reserved.

引用

页码：150 / 157

页数：8

共 20 条

[1] The effect of bovine serum albumin on batch and continuous enzymatic cellulose hydrolysis mixed by stirring or shaking
Brethauer, Simone
Studer, Michael H.
Yang, Bin
Wyman, Charles E.
[J]. BIORESOURCE TECHNOLOGY, 2011, 102 (10) : 6295 - 6298
[2] Soybean protein as a cost-effective lignin-blocking additive for the saccharification of sugarcane bagasse
Florencio, Camila
Badino, Alberto C.
Farinas, Cristiane S.
[J]. BIORESOURCE TECHNOLOGY, 2016, 221 : 172 - 180
[3] Lignin triggers irreversible cellulase loss during pretreated lignocellulosic biomass saccharification
Gao, Dahai
Haarmeyer, Carolyn
Balan, Venkatesh
Whitehead, Timothy A.
Dale, Bruce E.
Chundawat, Shishir P. S.
[J]. BIOTECHNOLOGY FOR BIOFUELS, 2014, 7
[4] Adsorption of β-glucosidases in two commercial preparations onto pretreated biomass and lignin
Haven, Mai Ostergaard
Jorgensen, Henning
[J]. BIOTECHNOLOGY FOR BIOFUELS, 2013, 6
[5] Comparative secretome analyses of two Trichoderma reesei RUT-C30 and CL847 hypersecretory strains
Herpoel-Gimbert, Isabelle
Margeot, Antoine
Dolla, Alain
Jan, Gwenael
Molle, Daniel
Lignon, Sabrina
Mathis, Hughes
Sigoillot, Jean-Claude
Monot, Frederic
Asther, Marcel
[J]. BIOTECHNOLOGY FOR BIOFUELS, 2008, 1 (1)
[6] Biomass recalcitrance: Engineering plants and enzymes for biofuels production
Himmel, Michael E.
Ding, Shi-You
Johnson, David K.
Adney, William S.
Nimlos, Mark R.
Brady, John W.
Foust, Thomas D.
[J]. SCIENCE, 2007, 315 (5813) : 804 - 807
[7] Effect of non-enzymatic proteins on enzymatic hydrolysis and simultaneous saccharification and fermentation of different lignocellulosic materials
Hui, Wang
Shinichi, Kobayashi
Kazuhiro, Mochidzuki
[J]. BIORESOURCE TECHNOLOGY, 2015, 190 : 373 - 380
[8] Integrated enzyme production lowers the cost of cellulosic ethanol
Johnson, Eric
[J]. BIOFUELS BIOPRODUCTS & BIOREFINING-BIOFPR, 2016, 10 (02): : 164 - 174
[9] The lignin present in steam pretreated softwood binds enzymes and limits cellulose accessibility
Kumar, Linoj
Arantes, Valdeir
Chandra, Richard
Saddler, Jack
[J]. BIORESOURCE TECHNOLOGY, 2012, 103 (01) : 201 - 208
[10] Lignocellulosic biomass for bioethanol production: Current perspectives, potential issues and future prospects
Limayem, Alya
Ricke, Steven C.
[J]. PROGRESS IN ENERGY AND COMBUSTION SCIENCE, 2012, 38 (04) : 449 - 467

← 1 2 →