Purification, in vitro antioxidant and in vivo anti-aging activities of exopolysaccharides by Agrocybe cylindracea

被引：44
作者：
Zhao, Huajie [1 ]
Li, Juan [2 ]
Zhang, Jianjun [1 ]
Wang, Xiuxiu [1 ]
Hao, Long [1 ]
Jia, Le [1 ]
机构：
[1] Shandong Agr Univ, Coll Life Sci, Tai An 271018, Shandong, Peoples R China
[2] Chinese Acad Agr Sci, Inst Agr Resources & Reg Planning, Minist Agr, Key Lab Plant Nutr & Fertilizer, Beijing 100081, Peoples R China
来源：
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | 2017年 / 102卷
关键词：
Agrocybe cylindraceas; Anti-aging activities; Antioxidant activities; Exopolysaccharides; HEPATOPROTECTIVE ACTIVITIES; LIPID-PEROXIDATION; SUBMERGED CULTURE; FREE-RADICALS; D-GALACTOSE; POLYSACCHARIDES; MICE; OPTIMIZATION; SENESCENCE; DISEASE;
D O I：
10.1016/j.ijbiomac.2017.04.039
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The in vitro antioxidant and in vivo anti-aging activities in D-galactose-induced aging mice of exopolysaccharides (EPS) and its fractions (EPS-1, EPS-2 and EPS-3) by Agrocybe cylindraceas were investigated presently. The scavenging effects of EPS-2 on hydroxyl (HO center dot) and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals were 97.1 +/- 4.25% and 90.23 +/- 13.25%, while the reducing power reached 1.18 +/- 0.08 at 120 mg/L, which were higher than EPS, EPS-1 and EPS-3 at 1000 mg/L. The EPS showed potential anti-aging activities via reducing the contents of malonaldehyde (MDA) and total cholesterol (TC), as well as increasing the activities of superoxide dismutase (SOD) activities, glutathione peroxidase (GSH-Px) activities, and total antioxidant capacity (T-AOC). Additionally, the monosaccharide analysis showed EPS contained rhamnose and glucose with the molar ratio of 2.8:1. These results demonstrated that EPS could be used as functional foods and natural drugs on preventing and mitigating the aging process. (C) 2017 Elsevier B.V. All rights reserved.
引用
收藏
页码：351 / 357
页数：7
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