Molecular and functional characterization of a cyclophilin with antifungal activity from Chinese cabbage

被引：32
作者：
Lee, Jung Ro
Park, Seong-Cheol
Kim, Jin-Young
Lee, Seung Sik
Park, Yoonkyung
Cheong, Gang-Won
Hahm, Kyung-Soo
Lee, Sang Yeol [1 ]
机构：
[1] Gyeongsang Natl Univ, Environm Biotechnol Natl Core Res Ctr, Jinju 660701, South Korea
[2] Gyeongsang Natl Univ, Div Appl Life Sci, BK21 Program, Jinju 660701, South Korea
[3] Chosun Univ, RCPM, Kwangju 501759, South Korea
[4] Chosun Univ, Dept Med, Kwangju 501759, South Korea
[5] Chosun Univ, Biotechnol & BK21 Res Team Prot Activ Control, Kwangju 501759, South Korea
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2007年 / 353卷 / 03期
关键词：
antifungal activity; Chinese cabbage; cyclophilin; peptidyl-prolyl cis-trans isomerase;
D O I：
10.1016/j.bbrc.2006.12.102
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
An antifungal protein that inhibits the growth of filamentous fungal pathogens was isolated from Chinese cabbage (Brassica campestris L. ssp. pekinensis) by affinity chromatography on Affi-gel blue gel and ion exchange chromatography on CM-Sepharose. The N-terminal amino acid sequence of the protein was highly homologous to that of plant cyclophilins and consequently the protein was denoted as C-CyP. To understand the antifungal activity of C-CyP, we isolated a cDNA encoding its gene from a Chinese cabbage leaf cDNA library. The Chinese cabbage genome bears more than one C-CyP gene copy and C-CyP mRNA is highly expressed in all tissues except the seeds. Recombinant C-CyP catalyzed the cis-trans inter-conversion of the Ala-Pro bond of the substrate, which indicates this protein has peptidyl-prolyl cis-trans isomerase activity. It also inhibited the growth of several fungal pathogens. (c) 2006 Elsevier Inc. All rights reserved.
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收藏
页码：672 / 678
页数：7
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