Antigen-specific CD8(+) T cell subset distribution in lymph nodes draining the site of herpes simplex virus infection

被引:36
作者
Cose, SC
Jones, CM
Wallace, ME
Heath, WR
Carbone, FR
机构
[1] ALFRED HOSP,MONASH MED SCH,DEPT PATHOL & IMMUNOL,PRAHRAN,VIC 3181,AUSTRALIA
[2] WALTER & ELIZA HALL INST MED RES,MELBOURNE,VIC 3050,AUSTRALIA
关键词
cytotoxic T lymphocyte; T cell receptor; herpes simplex virus; lymph node;
D O I
10.1002/eji.1830270927
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Inoculation with replicating virus leads to an increase in T cell numbers within lymph nodes that drain the site of infection. This increase has been associated with a nonspecific proliferation of bystander cells, with only a minority thought to be directed to the infectious agent. Such an assumption is largely based on precursor cytotoxic T lymphocyte (CTL) estimations using limiting dilution analysis. Recently, studies using more advanced molecular approaches have suggested that such functionally derived precursor frequencies considerably underestimate the proportion of T cells specific for the antigen under investigation. We have defined T cell receptor sequences characteristic of CTL populations directed to a dominant determinant of the herpes simplex virus (HSV) glycoprotein B (gB). In this investigation, we used this receptor signature as a probe to directly monitor changes occurring within lymph nodes draining the sites of active infection with HSV. We found that although lymph node CD8(+) T cell numbers increase as a consequence of HSV infection, the majority of these cells are small resting cells that are not enriched for gB-specific receptors. In contrast, a significant proportion of activated T cells are highly enriched for CTL bearing gB-specific receptors. Our results are therefore consistent with a nonspecific migration of CTL precursors into the lymph nodes draining the site of infection, followed by the activation and proliferation of the antigen-specific subset that normally makes up a small proportion of the naive T cell repertoire.
引用
收藏
页码:2310 / 2316
页数:7
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