Augmentation of 11β-hydroxysteroid LPS-activated J774.1 macrophages dehydrogenase type 1 in -: Role of 11β-HSD1 in pro-inflammatory properties in macrophages

被引：40

作者：

Ishii, Takako ^{[1
]}

Masuzaki, Hiroaki ^{[1
]}

Tanaka, Tomohiro ^{[1
]}

Arai, Naoki ^{[1
]}

Yasue, Shintaro ^{[1
]}

Kobayashi, Nozomi ^{[1
]}

Tomita, Tsutomu ^{[1
]}

Noguchi, Michio ^{[1
]}

Fujikura, Junji ^{[1
]}

Ebihara, Ken ^{[1
]}

Hosoda, Kiminori ^{[1
]}

Nakao, Kazuwa ^{[1
]}

机构：

[1] Kyoto Univ, Grad Sch Med, Dept Med & Clin Sci, Div Endocrinol & Metab, Kyoto 6068507, Japan

来源：

FEBS LETTERS | 2007年 / 581卷 / 03期

基金：

日本科学技术振兴机构;

关键词：

11; beta-HSD1; macrophage; inflammation; metabolic syndrome; inhibitor; adipocyte;

D O I：

10.1016/j.febslet.2006.11.032

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Macrophage infiltration in obese adipose tissue provokes local inflammation and insulin resistance. Evidence has accumulated that activation of 11 beta-HSD1 in adipocytes is critically involved in dysfunction of adipose tissue. However, the potential role of 11 beta-HSD1 in macrophages still remains unclear. We here demonstrate that a murine macrophage cell line, J774.1 cells expressed 11 beta-HSD1 mRNA and reductase activity, both of which were augmented by lipopolysaccharide (LPS)-induced cell activation. Three kinds of pharmacological inhibition of 11 beta-HSD1 in LPS-treated macrophages significantly suppressed the expression and secretion of interleukin I P, tumor necrosis factor a or monocyte chemoattractant protein 1, thereby highlighting a novel role of 11 beta-HSD1 in pro-inflammatory properties of activated macrophages. (c) 2006 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：349 / 354

页数：6

共 31 条

[1] Selective inhibition of 11β-hydroxysteroid dehydrogenase type 1 improves hepatic insulin sensitivity in hyperglycemic mice strains [J].

Alberts, P ;

Nilsson, C ;

Selén, G ;

Engblom, LOM ;

Edling, NHM ;

Norling, S ;

Klingström, G ;

Larsson, C ;

Forsgren, M ;

Ashkzari, M ;

Nilsson, CE ;

Fiedler, M ;

Bergqvist, E ;

Öhman, B ;

Björkstrand, E ;

Abrahmsén, LB .

ENDOCRINOLOGY, 2003, 144 (11) :4755-4762

[2] Effects of the 11β-hydroxysteroid dehydrogrenase inhibitor carbenoxolone on insulin sensitivity in men with type 2 diabetes [J].

Andrews, RC ;

Rooyackers, O ;

Walker, BR .

JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM, 2003, 88 (01) :285-291

[3]

BARBER AE, 1993, J IMMUNOL, V150, P1999

[4] Does central obesity reflect ''Cushing's disease of the omentum''? [J].

Bujalska, IJ ;

Kumar, S ;

Stewart, PM .

LANCET, 1997, 349 (9060) :1210-1213

[5] Prevalence of the metabolic syndrome among US adults - Findings from the Third National Health and Nutrition Examination Survey [J].

Ford, ES ;

Giles, WH ;

Dietz, WH .

JAMA-JOURNAL OF THE AMERICAN MEDICAL ASSOCIATION, 2002, 287 (03) :356-359

[6] Local amplification of Glucocorticoids by 11β-hydroxysteroid dehydrogenase type 1 promotes macrophage phagocytosis of apoptotic leukocytes [J].

Gilmour, James S. ;

Coutinho, Agnes E. ;

Cailhier, Jean-François ;

Man, Tak Yung ;

Clay, Michael ;

Thomas, Graham ;

Harris, Hayley J. ;

Mullins, John J. ;

Seckl, Jonathan R. ;

Savill, John S. ;

Chapman, Karen E. .

JOURNAL OF IMMUNOLOGY, 2006, 176 (12) :7605-7611

[7] Intracellular regeneration of glucocorticoids by 11β-hydroxysteroid dehydrogenase (11β-HSD)-1 plays a key role in regulation of the hypothalamic-pituitary-adrenal axis:: Analysis of 11β-HSD-1-deficient mice [J].

Harris, HJ ;

Kotelevtsev, Y ;

Mullins, JJ ;

Seckl, JR ;

Holmes, MC .

ENDOCRINOLOGY, 2001, 142 (01) :114-120

[8] 11β-HSD1 inhibition ameliorates metabolic syndrome and prevents progression of atherosclerosis in mice [J].

Hermanowski-Vosatka, A ;

Balkovec, JM ;

Cheng, K ;

Chen, HY ;

Hernandez, M ;

Koo, GC ;

Le Grand, CB ;

Li, ZH ;

Metzger, JM ;

Mundt, SS ;

Noonan, H ;

Nunes, CN ;

Olson, SH ;

Pikounis, B ;

Ren, N ;

Robertson, N ;

Schaeffer, JM ;

Shah, K ;

Springer, MS ;

Strack, AM ;

Strowski, M ;

Wu, K ;

Wu, TJ ;

Xiao, JY ;

Zhang, BB ;

Wright, SD ;

Thieringer, R .

JOURNAL OF EXPERIMENTAL MEDICINE, 2005, 202 (04) :517-527

[9]

Hoshino K, 1999, J IMMUNOL, V162, P3749

[10] Active site variability of type 1 11β-hydroxysteroid dehydrogenase revealed by selective inhibitors and cross-species comparisons [J].

Hult, M ;

Shafqat, N ;

Elleby, B ;

Mitschke, D ;

Svensson, S ;

Forsgren, M ;

Barf, T ;

Vallgårda, J ;

Abrahmsen, L ;

Oppermann, U .

MOLECULAR AND CELLULAR ENDOCRINOLOGY, 2006, 248 (1-2) :26-33

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