A versatile and scalable two-step ion-exchange chromatography process for the purification of recombinant adeno-associated virus serotypes-2 and-5

被引：64

作者：

Brument, N ^{[1
]}

Morenweiser, R ^{[1
]}

Blouin, V ^{[1
]}

Toublanc, E ^{[1
]}

Raimbaud, I ^{[1
]}

Chérel, Y ^{[1
]}

Folliot, S ^{[1
]}

Gaden, F ^{[1
]}

Boulanger, P ^{[1
]}

Kroner-Lux, G ^{[1
]}

Moullier, P ^{[1
]}

Rolling, F ^{[1
]}

Salvetti, A ^{[1
]}

机构：

[1] CHU Nantes, INSERM, ERM 0105, Lab Therapie Gen,Hotel Dieu, F-44035 Nantes, France

来源：

MOLECULAR THERAPY | 2002年 / 6卷 / 05期

关键词：

AAV purification; ion-exchange chromatography;

D O I：

10.1016/S1525-0016(02)90719-7

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Here we describe the development of a two-step chromatography process based on the use of ion-exchange resins for the purification of recombinant adeno-associated virus (rAAV) serotypes-2 and -5. In vitro and in vivo results demonstrate that this method, which does not require any prepurification step of the cell lysate, can be applied to obtain highly pure rAAV2 and rAAV5 stocks. As such, this procedure can be easily transferred in vector cores and also scaled up, allowing the direct comparison of these two, and potentially other, AAV serotypes in large animal models.

引用

页码：678 / 686

页数：9

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