Role of Heparanase-1 in Gastric Carcinoma Invasion

被引：1

作者：

Xu Yingying ^{[1
]}

Zhang Yong ^{[1
]}

Wang Zhenning ^{[1
]}

Zhang Xue ^{[2
,3
]}

Jiang Li ^{[2
,3
]}

Luo Yang ^{[2
,3
]}

Xu Huimian ^{[1
]}

机构：

[1] China Med Univ, Hosp 1, Dept Surg Oncol & Gen Surg, Shenyang 110001, Liaoning Prov, Peoples R China

[2] China Med Univ, Res Ctr Med Genom, Shenyang 110001, Peoples R China

[3] China Med Univ, MOH Key Lab Cell Biol, Shenyang 110001, Peoples R China

来源：

ASIAN PACIFIC JOURNAL OF CANCER PREVENTION | 2009年 / 10卷 / 01期

基金：

美国国家科学基金会;

关键词：

Heparanase-1; endoglycosidase; siRNA; invasion; metastasis; TUMOR INVASION; MAMMALIAN HEPARANASE; EXPRESSION; METASTASIS; CLONING; SULFATE; CELLS; GENE; CANCER;

D O I：

暂无

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Purpose: The heparan sulfate-degrading endoglycosidase may mediate tumor invasion and metastasis. It is known that heparanase-1 (HPA-1) plays an important role in cleaving heparan sulfate. In this study we investigated its potential role in gastric carcinoma malignant behaviour. Methods: To assess the role of HPA-1, we suppressed its expression using small interfering RNA (siRNA). The human heparanase-1 specific siRNA was transfected to the human gastric carcinoma cell line SGC7901. The effect of gene silencing was assessed by Real-Time PCR and Western Blot. The invasion potential of the cells was evaluated using an in vitro cell invasion model system. Results: The HPA-1 specific siRNA significantly suppressed expression of heparanase-1 in SGC7901 cells. Invasion after RNAi treatment through membranes in a model system was significantly decreased. Conclusion: HPA-1 facilitates gastric carcinoma invasion by up-regulating endoglycosidase activity in tumor cells.

引用

页码：151 / 154

页数：4

共 20 条

[1] COMPARATIVE-ANALYSIS OF THE ABILITY OF LEUKOCYTES, ENDOTHELIAL-CELLS AND PLATELETS TO DEGRADE THE SUBENDOTHELIAL BASEMENT-MEMBRANE - EVIDENCE FOR CYTOKINE DEPENDENCE AND DETECTION OF A NOVEL SULFATASE [J].