Expression, purification, and characterization of exo-β-D-glucosaminidase of Aspergillus sp CJ22-326 from Escherichia coli

被引：7

作者：

Li, Songlin ^{[1
]}

Wang, Chen ^{[2
]}

Xia, Wenshui ^{[1
]}

机构：

[1] Jiangnan Univ, State Key Lab Food Sci & Technol, Sch Food Sci & Technol, Wuxi 214122, Jiangsu, Peoples R China

[2] Chinese Acad Sci, Mol Cell Biol Lab, Inst Biochem & Cell Biol, Shanghai Inst Biol Sci, Shanghai 200031, Peoples R China

来源：

CARBOHYDRATE RESEARCH | 2009年 / 344卷 / 08期

关键词：

Aspergillus; Exo-beta-D-glucosaminidase; Gene cloning; His-Tag purification; CHITOSANASE;

D O I：

10.1016/j.carres.2009.02.026

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

An exo-beta-D-glucosaminidase gene was cloned from Aspergillus sp. CJ22-326 and expressed in Escherichia coli. The purified protein showed an exo-chitosanase activity in a viscosimetric assay and TLC analysis. This is the first report on cloning of a gene encoding an Aspergillus sp. exo-beta-D-glucosaminidase. (C) 2009 Published by Elsevier Ltd.

引用

页码：1046 / 1049

页数：4

共 25 条

[1] Removal of poly-histidine fusion tags from recombinant proteins purified by expanded bed adsorption [J].