Leptin and leptin-receptor polymorphisrns in fertile and infertile men

被引：13

作者：

Khosropour, Saeid ^{[1
]}

Hamidi, Maryam ^{[2
]}

Fattahi, Amir ^{[3
]}

Khodadadi, Lraj ^{[1
]}

Karami, Manoochehr ^{[4
,5
]}

Fazilati, Mohammad ^{[2
]}

Vaisi-Raygani, Asad ^{[6
]}

Tavilani, Heidar ^{[7
]}

机构：

[1] Hamadan Univ Med Sci, Sch Med, Dept Biochem, Hamadan, Iran

[2] Payam E Noor Univ, Dept Biol, Esfahan, Iran

[3] Tabriz Univ Med Sci, Womens Reprod Hlth Res Ctr, Tabriz, Iran

[4] Hamadan Univ Med Sci, Sch Publ Hlth, Social Determinants Hlth Res Ctr, Hamadan, Iran

[5] Hamadan Univ Med Sci, Sch Publ Hlth, Dept Epidemiol, Hamadan, Iran

[6] Kermanshah Univ Med Sci, Fertil & Infertil Res Ctr, Kermanshah, Iran

[7] Hamadan Univ Med Sci, Urol & Nephrol Res Ctr, Shaheed Fahmideh Ave, Hamadan, Iran

来源：

SYSTEMS BIOLOGY IN REPRODUCTIVE MEDICINE | 2017年 / 63卷 / 01期

关键词：

Genetic polymorphism; infertility; leptin; leptin receptor; male; reproduction; G-2548A POLYMORPHISM; GENE-EXPRESSION; OBESITY; ASSOCIATION; MUTATION; GNRH;

D O I：

10.1080/19396368.2016.1258741

中图分类号：

R69 [泌尿科学（泌尿生殖系疾病）];

学科分类号：

摘要：

The association of leptin (LEP) -2548G/A and/or leptin receptor (LEPR) Gln223Arg polyrnorphisms with male infertility and plasma FSH, LH and testosterone (T) levels was examined: The genotypes and allele frequency distributions of LEP -2548G/A and LEPR Gln223Arg polymorphisms were investigated in 150 fertile and 150 infertile men by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Also, plasma levels of FSH, LH, and T were measured using commercial ELISA kits. Frequencies of AA, AG and GG genotypes of LEP-2548G/A polymorphism were statistically different in fertile and infertile men (0=0.012). The AG genotype showed a protective effect which could decrease risk of male:infertility about 3 fold (p = 0.004).,We did not observe any differences infrequencies of LEPR Gln223Arg alleles and genotypes between groups (p > .0.05). Sperm counts from infertile men with the AG and GG genotypes of the LEP polymorphism were significantly higher than AA genotype (p<0.05). Moreover, infertile men who carried the RR genotype of LEPR showed a statistically higher percentage of sperm with progressive motility than individuals with other genotypes (p = 0:004). There was no correlation between different combinations of LEP and LEPR genotypes and LH, FSH; and I levels (0 > 0.05). Our study suggests that the LEP -2548G/A polymorphism may play a role in male fertility and the AG genotype may have a protective effect through increasing sperm counts. The distribution of genotypes of LEP -2548G/A polymorphism are different in fertile and infertile males and, may be a useful,tool in evaluation of male infertility.

引用

页码：7 / 14

页数：8

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