Leptin and leptin-receptor polymorphisrns in fertile and infertile men

被引：13
作者：
Khosropour, Saeid [1 ]
Hamidi, Maryam [2 ]
Fattahi, Amir [3 ]
Khodadadi, Lraj [1 ]
Karami, Manoochehr [4 ,5 ]
Fazilati, Mohammad [2 ]
Vaisi-Raygani, Asad [6 ]
Tavilani, Heidar [7 ]
机构：
[1] Hamadan Univ Med Sci, Sch Med, Dept Biochem, Hamadan, Iran
[2] Payam E Noor Univ, Dept Biol, Esfahan, Iran
[3] Tabriz Univ Med Sci, Womens Reprod Hlth Res Ctr, Tabriz, Iran
[4] Hamadan Univ Med Sci, Sch Publ Hlth, Social Determinants Hlth Res Ctr, Hamadan, Iran
[5] Hamadan Univ Med Sci, Sch Publ Hlth, Dept Epidemiol, Hamadan, Iran
[6] Kermanshah Univ Med Sci, Fertil & Infertil Res Ctr, Kermanshah, Iran
[7] Hamadan Univ Med Sci, Urol & Nephrol Res Ctr, Shaheed Fahmideh Ave, Hamadan, Iran
来源：
SYSTEMS BIOLOGY IN REPRODUCTIVE MEDICINE | 2017年 / 63卷 / 01期
关键词：
Genetic polymorphism; infertility; leptin; leptin receptor; male; reproduction; G-2548A POLYMORPHISM; GENE-EXPRESSION; OBESITY; ASSOCIATION; MUTATION; GNRH;
D O I：
10.1080/19396368.2016.1258741
中图分类号：
R69 [泌尿科学（泌尿生殖系疾病）];
学科分类号：
摘要：
The association of leptin (LEP) -2548G/A and/or leptin receptor (LEPR) Gln223Arg polyrnorphisms with male infertility and plasma FSH, LH and testosterone (T) levels was examined: The genotypes and allele frequency distributions of LEP -2548G/A and LEPR Gln223Arg polymorphisms were investigated in 150 fertile and 150 infertile men by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Also, plasma levels of FSH, LH, and T were measured using commercial ELISA kits. Frequencies of AA, AG and GG genotypes of LEP-2548G/A polymorphism were statistically different in fertile and infertile men (0=0.012). The AG genotype showed a protective effect which could decrease risk of male:infertility about 3 fold (p = 0.004).,We did not observe any differences infrequencies of LEPR Gln223Arg alleles and genotypes between groups (p > .0.05). Sperm counts from infertile men with the AG and GG genotypes of the LEP polymorphism were significantly higher than AA genotype (p<0.05). Moreover, infertile men who carried the RR genotype of LEPR showed a statistically higher percentage of sperm with progressive motility than individuals with other genotypes (p = 0:004). There was no correlation between different combinations of LEP and LEPR genotypes and LH, FSH; and I levels (0 > 0.05). Our study suggests that the LEP -2548G/A polymorphism may play a role in male fertility and the AG genotype may have a protective effect through increasing sperm counts. The distribution of genotypes of LEP -2548G/A polymorphism are different in fertile and infertile males and, may be a useful,tool in evaluation of male infertility.
引用
收藏
页码：7 / 14
页数：8
相关论文
共 36 条
[1]   Selective Deletion of Leptin Receptors in Gonadotropes Reveals Activin and GnRH-Binding Sites as Leptin Targets in Support of Fertility [J].
Akhter, Noor ;
CarlLee, Tyler ;
Syed, Mohsin M. ;
Odle, Angela K. ;
Cozart, Michael A. ;
Haney, Anessa C. ;
Allensworth-James, Melody L. ;
Benes, Helen ;
Childs, Gwen V. .
ENDOCRINOLOGY, 2014, 155 (10) :4027-4042
[2]   Male Fertility and Obesity: Are Ghrelin, Leptin and Glucagon-Like Peptide-1 Pharmacologically Relevant? [J].
Alves, Marco G. ;
Jesus, Tito T. ;
Sousa, Mario ;
Goldberg, Erwin ;
Silva, Branca M. ;
Oliveira, Pedro F. .
CURRENT PHARMACEUTICAL DESIGN, 2016, 22 (07) :783-791
[3]   Leptin and leptin receptor in pig spermatozoa: evidence of their involvement in sperm capacitation and survival [J].
Aquila, Saveria ;
Rago, Vittoria ;
Guido, Carmela ;
Casaburi, Ivan ;
Zupo, Silvia ;
Carpino, Amalia .
REPRODUCTION, 2008, 136 (01) :23-32
[4]   Leptin Receptor Gene Polymorphisms and the Risk of Non-Alcoholic Fatty Liver Disease and Coronary Atherosclerosis in the Chinese Han Population [J].
Bai-Quan An ;
Lu, Lin-Lin ;
Yuan, Chen ;
Xin, Yong-Ning ;
Xuan, Shi-Ying .
HEPATITIS MONTHLY, 2016, 16 (04)
[5]   A mutation in the human leptin receptor gene causes obesity and pituitary dysfunction [J].
Clément, K ;
Vaisse, C ;
Lahlou, N ;
Cabrol, S ;
Pelloux, V ;
Cassuto, D ;
Gourmelen, M ;
Dina, C ;
Chambaz, J ;
Lacorte, JM ;
Basdevant, A ;
Bougneres, P ;
Lebouc, Y ;
Froguel, P ;
Guy-Grand, B .
NATURE, 1998, 392 (6674) :398-401
[6]   Leptin's actions on the reproductive axis: Perspectives and mechanisms [J].
Cunningham, MJ ;
Clifton, DK ;
Steiner, RA .
BIOLOGY OF REPRODUCTION, 1999, 60 (02) :216-222
[7]   Expression Profile of Leptin and Aromatase-P450 Genes and Association with Sperm Motility in Fresh Bovine Ejaculates [J].
Dhanoa J.K. ;
Mukhopadhyay C.S. ;
Arora J.S. ;
Kaur S. .
Proceedings of the National Academy of Sciences, India Section B: Biological Sciences, 2016, 86 (3) :611-616
[8]   Leptin exists in tubuli seminiferi and in seminal plasma [J].
Glander, HJ ;
Lammert, A ;
Paasch, U ;
Glasow, A ;
Kratzsch, J .
ANDROLOGIA, 2002, 34 (04) :227-233
[9]  
Gong DW, 1996, J BIOL CHEM, V271, P3971
[10]   Patterns of Corpora Lutea Growth and Progesterone Secretion in Sows with Thrifty Genotype and Leptin Resistance due to Leptin Receptor Gene Polymorphisms (Iberian Pig) [J].
Gonzalez-Anover, P. ;
Encinas, T. ;
Torres-Rovira, L. ;
Sanz, E. ;
Pallares, P. ;
Ros, J. M. ;
Gomez-Izquierdo, E. ;
Sanchez-Sanchez, R. ;
Gonzalez-Bulnes, A. .
REPRODUCTION IN DOMESTIC ANIMALS, 2011, 46 (06) :1011-1016
1234