Semisynthesis of a functional K+ channel

被引：46
作者：
Valiyaveetil, FI
Sekedat, M
Muir, TW
MacKinnon, R
机构：
[1] Rockefeller Univ, Lab Synth Prot Chem, New York, NY 10021 USA
[2] Rockefeller Univ, Lab Mol Neurobiol & Biophys, New York, NY 10021 USA
来源：
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2004年 / 43卷 / 19期
关键词：
ion channels; peptides; potassium; protein engineering;
D O I：
10.1002/anie.200453849
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
Selective ion-conduction properties in lipid membranes have been found for a semisynthetic KcsA K+ channel containing a mutation (A98G) that allows the channel to open. Thus, it is now possible to study ion conduction in a K+ channel with chemical modification of the main-chain atoms of the selectivity filter. The picture shows the single-channel responses of the semisynthetic K+ channel and its structure.
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收藏
页码：2504 / 2507
页数：4
相关论文
共 12 条
[1]  
[Anonymous], 1992, Ionic Channels of Excitable Membranes Sunderland
[2]   Structural dynamics of the Streptomyces lividans K+ channel (SKC1): Oligomeric stoichiometry and stability [J].
Cortes, DM ;
Perozo, E .
BIOCHEMISTRY, 1997, 36 (33) :10343-10352
[3]   The structure of the potassium channel:: Molecular basis of K+ conduction and selectivity [J].
Doyle, DA ;
Cabral, JM ;
Pfuetzner, RA ;
Kuo, AL ;
Gulbis, JM ;
Cohen, SL ;
Chait, BT ;
MacKinnon, R .
SCIENCE, 1998, 280 (5360) :69-77
[4]   Crystal structure and mechanism of a calcium-gated potassium channel [J].
Jiang, YX ;
Lee, A ;
Chen, JY ;
Cadene, M ;
Chait, BT ;
MacKinnon, R .
NATURE, 2002, 417 (6888) :515-522
[5]   The open pore conformation of potassium channels [J].
Jiang, YX ;
Lee, A ;
Chen, JY ;
Cadene, M ;
Chait, BT ;
MacKinnon, R .
NATURE, 2002, 417 (6888) :523-526
[6]   The βγ subunits of G proteins gate a K+ channel by pivoted bending of a transmembrane segment [J].
Jin, TH ;
Peng, LY ;
Mirshahi, T ;
Rohacs, T ;
Chan, KW ;
Sanchez, R ;
Logothetis, DE .
MOLECULAR CELL, 2002, 10 (03) :469-481
[7]   CHEMICAL SYNTHESIS OF PEPTIDES AND PROTEINS [J].
KENT, SBH .
ANNUAL REVIEW OF BIOCHEMISTRY, 1988, 57 :957-989
[8]   Structural conservation in prokaryotic and eukaryotic potassium channels [J].
MacKinnon, R ;
Cohen, SL ;
Kuo, AL ;
Lee, A ;
Chait, BT .
SCIENCE, 1998, 280 (5360) :106-109
[9]   Semisynthesis of proteins by expressed protein ligation [J].
Muir, TW .
ANNUAL REVIEW OF BIOCHEMISTRY, 2003, 72 :249-289
[10]   Lipids in the structure, folding, and function of the KcsA K+ channel [J].
Valiyaveetil, FI ;
Zhou, YF ;
Mackinnon, R .
BIOCHEMISTRY, 2002, 41 (35) :10771-10777
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