Phytosulfokine alpha enhances microspore embryogenesis in both triticale and wheat

被引：27

作者：

Asif, Muhammad ^{[1
,2
]}

Eudes, Francois ^{[1
]}

Randhawa, Harpinder ^{[1
]}

Amundsen, Eric ^{[1
]}

Spaner, Dean ^{[2
]}

机构：

[1] Agr & Agri Food Canada, Lethbridge, AB T1J 4B1, Canada

[2] Univ Alberta, Dept Agr Food & Nutr Sci, Edmonton, AB T6G 2P5, Canada

来源：

PLANT CELL TISSUE AND ORGAN CULTURE | 2014年 / 116卷 / 01期

关键词：

Doubled haploid; Microspore culture; Nurse culture; Peptide; Phytosulfokine; Triticale; Wheat; PEPTIDE GROWTH-FACTOR; STIMULATES SOMATIC EMBRYOGENESIS; HORDEUM-VULGARE L; PLANT-CELLS; AESTIVUM L; SULFATED PENTAPEPTIDE; MESOPHYLL-CELLS; ANTHER CULTURE; PROLIFERATION; DIFFERENTIATION;

D O I：

10.1007/s11240-013-0379-y

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Isolated microspore culture (IMC) has been used to develop doubled haploid lines and to generate homozygous lines in a single generation for varietal development. Phytosulfokine has been previously used in promoting cell growth and embryo development in various systems. In this study, phytosulfokine alpha (PSK-alpha) supplemented IMC induction medium was evaluated in triticale and wheat cultivars, and the production of embryo-like structures (ELS), green and albino plants were recorded. In addition, the contribution of ovary co-culture was also evaluated in IMC on a NPB99 + 10F induction medium. Over a range of concentrations up to 10(-7)M, PSK-alpha yielded more ELS and green plants in wheat and triticale cultivars at the highest dose, when compared to the control. It also minimized albinism in wheat cultivars, but not in triticale. Interestingly, 10(-7)M PSK-alpha also supported the formation of a large number of embryos and a few green plants in the absence of nursing ovaries.

引用

页码：125 / 130

页数：6

共 39 条

[1] THE RESPONSE OF ANTHER CULTURE IN A GENETICALLY WIDE MATERIAL OF WINTER-WHEAT (TRITICUM-AESTIVUM L) [J].

ANDERSEN, SB ;

DUE, IK ;

OLESEN, A .

PLANT BREEDING, 1987, 99 (03) :181-186

[2]

[Anonymous], REL 9 2

[3]

Asif M, 2013, IN VITRO CE IN PRESS

[4] GROWTH + ORGANIZED DEVELOPMENT OF CULTURED CELLS .6. BEHAVIOR OF INDIVIDUAL CELLS ON NUTRIENT AGAR [J].

BLAKELY, LM .

AMERICAN JOURNAL OF BOTANY, 1964, 51 (07) :792-&

[5] Plastid differentiation during androgenesis in albino and non-albino producing cultivars of barley (Hordeum vulgare L.) [J].

Caredda, S ;

Doncoeur, C ;

Devaux, P ;

Sangwan, RS ;

Clément, C .

SEXUAL PLANT REPRODUCTION, 2000, 13 (02) :95-104

[6]

Chen DeXi Chen DeXi, 2010, Southwest China Journal of Agricultural Sciences, V23, P1447

[7] Peptide growth factor phytosulfokine-α contributes to the pollen population effect [J].

Chen, YF ;

Matsubayashi, Y ;

Sakagami, Y .

PLANTA, 2000, 211 (05) :752-755

[8] PRODUCTION OF LARGE NUMBER OF DOUBLED HAPLOID PLANTS FROM BARLEY ANTHERS PRETREATED WITH HIGH-CONCENTRATIONS OF MANNITOL [J].

CISTUE, L ;

RAMOS, A ;

CASTILLO, AM ;

ROMAGOSA, I .

PLANT CELL REPORTS, 1994, 13 (12) :709-712

[9]

Clément C, 2005, BIOTECHNOL AGRIC FOR, V56, P53

[10]

Eun C-H., 2003, Plant Biotechnol, V20, P195, DOI DOI 10.5511/PLANTBIOTECHNOLOGY.20.195

← 1 2 3 4 →