A rapid and precise method for measuring plasma apoE-rich HDL using polyethylene glycol and cation-exchange chromatography: a pilot study on the clinical significance of apoE-rich HDL measurements

被引：5

作者：

Ikeda, Toru ^{[1
]}

Shinohata, Ryoko ^{[1
]}

Murakami, Masaaki ^{[2
]}

Hina, Kazuyoshi ^{[2
]}

Kamikawa, Shigeshi ^{[2
]}

Hirohata, Satoshi ^{[1
]}

Kusachi, Shozo ^{[2
]}

Tamura, Arisa ^{[1
]}

Usui, Shinichi ^{[1
]}

机构：

[1] Okayama Univ, Grad Sch Hlth Sci, Dept Med Technol, Okayama, Japan

[2] Okayama Heart Clin, Okayama, Japan

来源：

CLINICA CHIMICA ACTA | 2017年 / 465卷

关键词：

HDL subfraction; Atherogenic lipoprotein; Chromatography; HIGH-DENSITY-LIPOPROTEIN; CORONARY-HEART-DISEASE; APOLIPOPROTEIN-E; ANTIINFLAMMATORY PROPERTIES; CHOLESTEROL LEVELS; BINDING; QUANTIFICATION; SUBPOPULATIONS; METABOLISM; PROTECTION;

D O I：

10.1016/j.cca.2016.12.016

中图分类号：

R446 [实验室诊断]; R-33 [实验医学、医学实验];

学科分类号：

1001 ;

摘要：

Background: High-density lipoprotein (HDL) containing apolipoprotein E (apoE-rich HDL) represents only a small portion of plasma HDL. Reliable methods for determining and isolating apoE-rich HDL have not been well studied. Methods: We established a novel analytical method for apoE-rich HDL using polyethylene glycol and a cation-exchange column (PEG-column method). Furthermore, we examined biochemical correlates of apoE-rich HDL-cholesterol (HDL-C) in 36 patients who underwent coronary computed tomographic angiography. Results: Our PEG-column method demonstrated high reproducibility (coefficient of variation <3.52%) and linearity up to 15 mg/dl for apoE-rich HDL-C concentrations. Isolated apoE-rich HDL exhibited a larger diameter (14.8 nm) than apoE-poor HDL (10.8 nm) and contained both apoE and apoA-I. ApoE-rich HDL-C concentrations correlated significantly with triglycerides (r(s) = -0.646), LDL size (r(s) = 0.472), adiponectin (r(s) = 0.476), and other lipoprotein components. No significant correlation was obtained with the coronary calcium score. Multiple regression analysis revealed that plasma triglycerides and adiponectin concentrations remained significant independent predictors of apoE-rich (adjusted R-2 = 0.486) but not apoE-poor HDL-C. Conclusions: The PEG-column method demonstrated, to various degrees, significant correlations between HDL subtractions and several lipid-related biomarkers involved in an atherogenic lipoprotein profile. Our separation technique for apoE-rich HDL is useful to clarify the role of apoE-rich HDL in atherosclerosis. (C) 2016 Elsevier B.V. All rights reserved.

引用

页码：112 / 118

页数：7