Single-cell analysis of germinal-center B cells informs on lymphoma cell of origin and outcome

被引：124

作者：

Holmes, Antony B. ^{[1
]}

Corinaldesi, Clarissa ^{[1
]}

Shen, Qiong ^{[1
]}

Kumar, Rahul ^{[1
]}

Compagno, Nicolo ^{[1
]}

Wang, Zhong ^{[2
]}

Nitzan, Mor ^{[7
]}

Grunstein, Eli ^{[3
]}

Pasqualucci, Laura ^{[1
,2
,6
]}

Dalla-Favera, Riccardo ^{[1
,2
,4
,5
,6
]}

Basso, Katia ^{[1
,2
]}

机构：

[1] Columbia Univ, Inst Canc Genet, New York, NY 10027 USA

[2] Columbia Univ, Dept Pathol & Cell Biol, New York, NY 10027 USA

[3] Columbia Univ, Dept Otolaryngol Head & Neck Surg, New York, NY USA

[4] Columbia Univ, Dept Microbiol & Immunol, New York, NY 10027 USA

[5] Columbia Univ, Dept Genet & Dev, New York, NY 10027 USA

[6] Columbia Univ, Herbert Irving Comprehens Canc Ctr, New York, NY 10027 USA

[7] Harvard Univ, Cambridge, MA 02138 USA

来源：

JOURNAL OF EXPERIMENTAL MEDICINE | 2020年 / 217卷 / 10期

基金：

美国国家卫生研究院;

关键词：

PLASMA-CELL; MEMORY B; TYROSINE PHOSPHORYLATION; CENTER SELECTION; CENTER LIGHT; CENTER DARK; IFN-GAMMA; EXPRESSION; DYNAMICS; TRANSCRIPTION;

D O I：

10.1084/jem.20200483

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

In response to T cell-dependent antigens, mature B cells are stimulated to form germinal centers (GCs), the sites of B cell affinity maturation and the cell of origin (COO) of most B cell lymphomas. To explore the dynamics of GC B cell development beyond the known dark zone and light zone compartments, we performed single-cell (sc) transcriptomic analysis on human GC B cells and identified multiple functionally linked subpopulations, including the distinct precursors of memory B cells and plasma cells. The gene expression signatures associated with these GC subpopulations were effective in providing a sc-COO for similar to 80% of diffuse large B cell lymphomas (DLBCLs) and identified novel prognostic subgroups of DLBCL.

引用

页数：26

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