Rac1 Modulates Excitatory Synaptic Transmission in Mouse Retinal Ganglion Cells

被引：10

作者：

Li, Ling-Zhu ^{[1
,2
]}

Yin, Ning ^{[1
,2
]}

Li, Xue-Yan ^{[1
,2
]}

Miao, Yanying ^{[1
,2
]}

Cheng, Shuo ^{[1
,2
]}

Li, Fang ^{[1
,2
]}

Zhao, Guo-Li ^{[1
,2
]}

Zhong, Shu-Min ^{[1
,2
]}

Wang, Xin ^{[1
,2
]}

Yang, Xiong-Li ^{[1
,2
]}

Wang, Zhongfeng ^{[1
,2
]}

机构：

[1] Fudan Univ, Dept Neurol, Inst Brain Sci, State Key Lab Med Neurobiol,Zhongshan Hosp, Shanghai 200032, Peoples R China

[2] Fudan Univ, MOE Frontiers Ctr Brain Sci, Zhongshan Hosp, Shanghai 200032, Peoples R China

来源：

NEUROSCIENCE BULLETIN | 2019年 / 35卷 / 04期

基金：

中国国家自然科学基金;

关键词：

Rac1; Retinal ganglion cell; Excitatory synaptic transmission; Dendrite; Dendritic spine; METABOTROPIC GLUTAMATE RECEPTORS; DENDRITIC ARBOR GROWTH; RHO-FAMILY GTPASES; PREFRONTAL CORTEX; AMPA RECEPTORS; ACTIVATION; NMDA; NEURONS; INHIBITION; PROTEIN;

D O I：

10.1007/s12264-019-00353-0

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Ras-related C3 botulinum toxin substrate 1 (Rac1), a member of the Rho GTPase family which plays important roles in dendritic spine morphology and plasticity, is a key regulator of cytoskeletal reorganization in dendrites and spines. Here, we investigated whether and how Rac1 modulates synaptic transmission in mouse retinal ganglion cells (RGCs) using selective conditional knockout of Rac1 (Rac1-cKO). Rac1-cKO significantly reduced the frequency of AMPA receptor-mediated miniature excitatory postsynaptic currents, while glycine/GABA(A) receptor-mediated miniature inhibitory postsynaptic currents were not affected. Although the total GluA1 protein level was increased in Rac1-cKO mice, its expression in the membrane component was unchanged. Rac1-cKO did not affect spine-like branch density in single dendrites, but significantly reduced the dendritic complexity, which resulted in a decrease in the total number of dendritic spine-like branches. These results suggest that Rac1 selectively affects excitatory synaptic transmission in RGCs by modulating dendritic complexity.

引用

页码：673 / 687

页数：15

共 80 条

[1] Modulation of dendritic spines and synaptic function by Rac1: A possible link to Fragile X syndrome pathology [J].