A Comparison of Labeling and Label-Free Mass Spectrometry-Based Proteomics Approaches

被引：196
|
作者：
Patel, Vibhuti J. [1 ]
Thalassinos, Konstantinos [1 ]
Slade, Susan E. [1 ]
Connolly, Joanne B. [2 ]
Crombie, Andrew [1 ]
Murrell, J. Colin [1 ]
Scrivens, James H. [1 ]
机构：
[1] Univ Warwick, Dept Biol Sci, Coventry CV4 7AL, W Midlands, England
[2] Waters Corp, Manchester M22 5PP, Lancs, England
来源：
JOURNAL OF PROTEOME RESEARCH | 2009年 / 8卷 / 07期
关键词：
proteomics; quantification; iTRAQ; label-free; methanotroph; mass spectrometry; COMPLEX PROTEIN MIXTURES; LIQUID-CHROMATOGRAPHY; GEL-ELECTROPHORESIS; ABSOLUTE QUANTIFICATION; QUANTITATIVE-ANALYSIS; SERUM; PRECURSOR; ABUNDANCE; SPECTRA; MS;
D O I：
10.1021/pr900080y
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
The proteome of the recently discovered bacterium Methyiocella silvestris has been characterized using three profiling and comparative proteomics approaches. The organism has been grown on two different substrates enabling variations in protein expression to be identified. The results obtained using the experimental approaches have been compared with respect to number of proteins identified, confidence in identification, sequence coverage and agreement of regulated proteins. The sample preparation, instrumental time and sample loading requirements of the differing experiments are compared and discussed. A preliminary screen of the protein regulation results for biological significance has also been performed.
引用
收藏
页码：3752 / 3759
页数：8
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