Effect of plant growth regulators for production of multiple shoots through in vitro culture of Yangambi and Champa variety of banana (Musa spp.)

Musa spp. cv. Yangambi Km5, an introduced variety and Champa, a local popular variety of banana available in the eastern belt of Odisha were taken as experimental source. Experiments were conducted taking shoot tip as explants for shoot proliferation of the two banana varieties. In vitro cultures were maintained by using Murashige and Skoog (MS)15 medium containing different concentration of cytokinins with combination of [benzylaminopurine (BAP), kinetin (Kin)], auxins [indole acetic acid (IAA) and naphthaleneacetic acid (NAA)] during multiplication stages. The best responses in terms of fresh weight, shoot length and number of shoots per explant were obtained from MS medium supplemented with 4mg/L BAP+1.0mg/L KIN + .5mg/L IAA in Yangambi and Champa variety.