Effects of different soil weights, storage times and extraction methods on soil phospholipid fatty acid analyses

被引：118

作者：

Wu, Yuping ^{[1
,2
,3
]}

Ding, Na ^{[1
,2
]}

Wang, Gang ^{[1
,2
]}

Xu, Jianming ^{[1
,2
]}

Wu, Jianjun ^{[1
,2
]}

Brookes, Philip C. ^{[3
]}

机构：

[1] Zhejiang Univ, Coll Environm & Nat Resource Sci, Hangzhou 310029, Zhejiang, Peoples R China

[2] Zhejiang Prov Key Lab Subtrop Soil & Plant Nutr, Hangzhou 310029, Zhejiang, Peoples R China

[3] Rothamsted Res, Dept Soil Sci, Harpenden AL5 2JQ, Herts, England

来源：

GEODERMA | 2009年 / 150卷 / 1-2期

基金：

中国国家自然科学基金;

关键词：

Phospholipid fatty acid (PLFA); Extraction method; Soil sample size; SPE tubes; Storage; MICROBIAL COMMUNITIES; LIPID PHOSPHATE; BIOMASS; PROFILES; PLFA; TEMPERATURE; FOREST;

D O I：

10.1016/j.geoderma.2009.02.003

中图分类号：

S15 [土壤学];

学科分类号：

0903 ; 090301 ;

摘要：

Phospholipid fatty acid (PLFA) analysis has become a popular and powerful method for characterizing soil microbial communities. However, little work has been done to compare different extraction procedures. In this study, the effects of single or consecutive extractions, varying soil weights, alternative solid phase extraction (SPE) tubes and a comparison of extractions from fresh soil and that from soil previously stored freeze-dried at -70 degrees C for 1 year were determined. About 90-95% of PLFAs were extracted by the method used in this paper. More individual PLFAs and changes in PLFA peak distributions (profiles) were found with increasing soil sample weights, which suggests that for each set of experiments, the soil weight should be constant. Home-made SPE tubes used in this study can give almost as good results as the commercial standard ones, which significantly decreased costs. The total PLFA concentrations decreased by more than 28% following storage of the freeze-dried soils for 1 year at -70 degrees C. The PLFA profiles also changed during storage over this period, suggesting that PLFA analyses are best done as soon as possible after sampling. (C) 2009 Elsevier B.V. All rights reserved.

引用

页码：171 / 178

页数：8

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