MEASUREMENT OF PLATELET-DERIVED MICROPARTICLE LEVELS USING AN ENZYME-LINKED IMMUNOSORBENT ASSAY IN POLYMYOSITIS AND DERMATOMYOSITIS PATIENTS

被引：17

作者：

Shirafuji, Toshihiko ^{[1
]}

Hamaguchi, Hirotoshi ^{[1
]}

Higuchi, Masatsugu ^{[1
]}

Kanda, Fumio ^{[1
]}

机构：

[1] Kobe Univ, Grad Sch Med, Div Neurol, Dept Internal Med,Chuo Ku, Kobe, Hyogo 6500017, Japan

来源：

MUSCLE & NERVE | 2009年 / 39卷 / 05期

关键词：

dermatomyositis; ELISA; idiopathic inflammatory myopathy; platelet-derived microparticle; polymyositis; CELL-ADHESION MOLECULE-1; INFLAMMATORY-BOWEL-DISEASE; INCREASED SERUM-LEVELS; IN-SITU EXPRESSION; SYSTEMIC-SCLEROSIS; E-SELECTIN; ACTIVATED PLATELETS; ENDOTHELIAL-CELLS; MYOPATHIES; SURFACE;

D O I：

10.1002/mus.21311

中图分类号：

R74 [神经病学与精神病学];

学科分类号：

摘要：

Platelet-derived microparticle (PDMP) levels were measured using an enzyme-linked immunosorbent assay (ELISA) to elucidate the role of platelet activation in patients with polymyositis or dermatomyositis (PM/DM). PDMP levels in active PM/DM patients (median 13.3 U/ml, interquartile range 9.9-20.7 U/ml = 16) and those in patients undergoing treatment (12.1 U/ml, 7.4-16.7 U/ml, n = 12) were significantly higher than in controls (6.5 U/ml 5.0-8.4 =U/ml, n = 26, vs. active, P = 0.0001; vs. treatment, P = 0.004). In a paired sampling study, PDMP decreased significantly after glucocorticoid treatment (P = 0.04). PDMP in the active PM/DM patients correlated significantly with serum C-reactive protein levels (r(s) = 0.67, P 0.01). These results suggest that platelets may play an important role in the inflammatory process, and that PDMP level could be a useful marker of inflammatory activity in PM/DM patients.

引用

页码：586 / 590

页数：5

共 43 条

[1] Elevated circulating platelet-derived microparticles in patients with active inflammatory bowel disease [J].

Andoh, A ;

Tsujikawa, T ;

Hata, K ;

Araki, Y ;

Kitoh, K ;

Sasaki, M ;

Yoshida, T ;

Fujiyama, Y .

AMERICAN JOURNAL OF GASTROENTEROLOGY, 2005, 100 (09) :2042-2048

[2]

[Anonymous], 2008, KOBE J MED SCI

[3] Mechanisms of cellular activation by platelet microparticles [J].

Barry, OP ;

FitzGerald, GA .

THROMBOSIS AND HAEMOSTASIS, 1999, 82 (02) :794-800

[4] Modulation of monocyte-endothelial cell interactions by platelet microparticles [J].

Barry, OP ;

Praticò, D ;

Savani, RC ;

FitzGerald, GA .

JOURNAL OF CLINICAL INVESTIGATION, 1998, 102 (01) :136-144

[5] Arachidonic acid in platelet microparticles up-regulates cyclooxygenase-2-dependent prostaglandin formation via a protein kinase C mitogen-activated protein kinase-dependent pathway [J].

Barry, OP ;

Kazanietz, MG ;

Praticò, D ;

FitzGerald, GA .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1999, 274 (11) :7545-7556

[6] POLYMYOSITIS AND DERMATOMYOSITIS .1. [J].

BOHAN, A ;

PETER, JB .

NEW ENGLAND JOURNAL OF MEDICINE, 1975, 292 (07) :344-347

[7] Circulating microparticles from patients with myocardial infarction cause endothelial dysfunction [J].

Boulanger, CM ;

Scoazec, A ;

Ebrahimian, T ;

Henry, P ;

Mathieu, E ;

Tedgui, A ;

Mallat, Z .

CIRCULATION, 2001, 104 (22) :2649-2652

[8]

Carota A, 1997, CLIN NEUROPATHOL, V16, P312

[9]

CARSON CW, 1993, J RHEUMATOL, V20, P809

[10] Endothelial and platelet activation in acute ischemic stroke and its etiological subtypes [J].

Cherian, P ;

Hankey, GJ ;

Eikelboom, JW ;

Thom, J ;

Baker, RI ;

McQuillan, A ;

Staton, J ;

Yi, QL .

STROKE, 2003, 34 (09) :2132-2137

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