The muscle protein Dok-7 is essential for neuromuscular synaptogenesis

被引：324

作者：

Okada, Kumiko

Inoue, Akane

Okada, Momoko

Murata, Yoji

Kakuta, Shigeru

Jigami, Takafumi

Kubo, Sachiko

Shiraishi, Hirokazu

Eguchi, Katsumi

Motomura, Masakatsu

Akiyama, Tetsu

Iwakura, Yoichiro

Higuchi, Osamu ^{[1
]}

Yamanashi, Yuji

机构：

[1] Tokyo Med & Dent Univ, Inst Med Res, Dept Cell Regulat, Tokyo 1138510, Japan

[2] Tokyo Med & Dent Univ, Sch Biomed Sci, Tokyo 1138510, Japan

[3] Univ Tokyo, Inst Med Sci, Ctr Med Expt, Tokyo 1088639, Japan

[4] Univ Tokyo, Inst Mol & Cellular Biosci, Lab Mol & Genet Informat, Tokyo 1130032, Japan

[5] Nagasaki Univ, Grad Sch Biomed Sci, Dept Internal Med 1, Nagasaki 8528501, Japan

来源：

SCIENCE | 2006年 / 312卷 / 5781期

关键词：

D O I：

10.1126/science.1127142

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The formation of the neuromuscular synapse requires muscle-specific receptor kinase (MuSK) to orchestrate postsynaptic differentiation, including the clustering of receptors for the neurotransmitter acetylcholine. Upon innervation, neural agrin activates MuSK to establish the postsynaptic apparatus, although agrin-independent formation of neuromuscular synapses can also occur experimentally in the absence of neurotransmission. Dok-7, a MuSK-interacting cytoplasmic protein, is essential for MuSK activation in cultured myotubes; in particular, the Dok-7 phosphotyrosine-binding domain and its target in MuSK are indispensable. Mice lacking Dok-7 formed neither acetylcholine receptor clusters nor neuromuscular synapses. Thus, Dok-7 is essential for neuromuscular synaptogenesis through its interaction with MuSK.

引用

页码：1802 / 1805

页数：4

共 25 条

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