Evaluation of transgenic lines of Indian mustard (Brassica juncea L. Czern and Coss) expressing synthetic cry1Ac gene for resistance to Plutella xylostella

被引：7

作者：

Kamble, Suchita ^{[1
]}

Hadapad, Ashok B. ^{[1
]}

Eapen, Susan ^{[1
]}

机构：

[1] Bhabha Atom Res Ctr, Nucl Agr & Biotechnol Div, Bombay 400085, Maharashtra, India

来源：

PLANT CELL TISSUE AND ORGAN CULTURE | 2013年 / 115卷 / 03期

关键词：

Agrobacterium-mediated transformation; B; juncea; Insect resistance; Synthetic cry1Ac gene; Transgenic plants; AGROBACTERIUM-MEDIATED TRANSFORMATION; BACILLUS-THURINGIENSIS CRY1C; DIAMONDBACK MOTH; STEM BORER; BT-GENES; RICE; PLANTS; MANAGEMENT; OLERACEA; GROWTH;

D O I：

10.1007/s11240-013-0364-5

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Transgenic lines of oil seed plant Brassica juncea expressing a synthetic cry1Ac gene were developed using Agrobacterium tumefaciens-mediated transformation. The integration of transgene cry1Ac in transformed plants was confirmed by Southern blot hybridization using a cry1Ac probe. Expression of transgene was analyzed using reverse transcription PCR. Inheritance of the hpt gene in transgenic plants showed the expected 3:1 Mendelian segregation ratio. An enzyme linked immuno sorbent assay revealed that Cry1Ac protein content in the leaves of transgenic plants (T-0, T-1 and T-2) ranged from 250 to 1,800 ng mg(-1) of total soluble protein. The insect bioassay study showed that transgenic plants were resistant to 2nd instar larvae of Plutella xylostella as compared to control plants and confirmed the enhanced insect resistance in Indian mustard.

引用

页码：321 / 328

页数：8

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