One-step PCR for the identification of multiple antimicrobial resistance in Neisseria gonorrhoeae

被引：6

作者：

Lawung, Ratana ^{[1
]}

Cherdtrakulkiat, Rungrot ^{[1
]}

Charoenwatanachokchai, Angkana ^{[2
]}

Nabu, Sunanta ^{[1
]}

Suksaluk, Wanvisa ^{[1
]}

Prachayasittikul, Virapong ^{[1
]}

机构：

[1] Mahidol Univ, Fac Med Technol, Dept Clin Microbiol, Bangkok 10700, Thailand

[2] Minist Publ Hlth, Dept Communicable Dis Control, Thai Bur AIDS TB & STD, Bangkok, Thailand

来源：

JOURNAL OF MICROBIOLOGICAL METHODS | 2009年 / 77卷 / 03期

关键词：

Neisseria gonorrhoeae; Multiplex PCR; Multiple antimicrobial resistance; SUSCEPTIBILITIES; HAEMOPHILUS; PREVALENCE; MUTATIONS; PLASMIDS; GYRA;

D O I：

10.1016/j.mimet.2009.03.009

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

One-step multiplex PCR was developed for the identification of gonococci and antimicrobial-resistant profiles. From forty Neisseria gonorrhoeae isolates, the penicillinase-producing N. gonorrhoeae (PPNG), the high-level tetracycline-resistant N. gonorrhoeae (TRNG), and the ciprofloxacin-resistant N. gonorrhoeae (CRNG) were successfully classified. Our method provides expediency and benefit to epidemiology and antimicrobial-resistance mobility with 100% sensitivity and specificity for gonococcal-detection. The detection limit was 500 CFU/reaction. (C) 2009 Elsevier B.V. All rights reserved.

引用

页码：323 / 325

页数：3

共 12 条

[1]

[Anonymous], 2006, M2A9 CLIN LAB STAND

[2] Improved green fluorescent protein by molecular evolution using DNA shuffling [J].

Crameri, A ;

Whitehorn, EA ;

Tate, E ;

Stemmer, WPC .

NATURE BIOTECHNOLOGY, 1996, 14 (03) :315-319

[3] Rapid detection of point mutations of the Neisseria gonorrhoeae gyrA gene associated with decreased susceptibilities to quinolones [J].

Deguchi, T ;

Yasuda, M ;

Nakano, M ;

Ozeki, S ;

Ezaki, T ;

Maeda, SI ;

Saito, I ;

Kawada, Y .

JOURNAL OF CLINICAL MICROBIOLOGY, 1996, 34 (09) :2255-2258

[4] NUCELOTIDE SEQUENCES OF THE TET(M) GENES FROM THE AMERICAN AND DUTCH TYPE TETRACYCLINE RESISTANCE PLASMIDS OF NEISSERIA-GONORRHOEAE [J].

GASCOYNEBINZI, DM ;

HERITAGE, J ;

HAWKEY, PM .

JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 1993, 32 (05) :667-676

[5]

Gerbase AC, 1998, SEX TRANSM INFECT, V74, pS12

[6]

Ison CA, 1998, LANCET, V351, P8

[7] Mutations in gyrA and parC in ciprofloxacin-resistant Neisseria gonorrhoeae in Kuala Lumpur, Malaysia [J].

Karunakaran, Rina ;

Sam, I-Ching .

JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 2007, 59 (04) :803-804

[8] Antimicrobial susceptibilities of strains of Neisseria gonorrhoeae in Bangkok, Thailand: 1994-1995 [J].

Knapp, JS ;

Wongba, C ;

Limpakarnjanarat, K ;

Young, NL ;

Parekh, MC ;

Neal, SW ;

Buatiang, A ;

Chitwarakorn, A ;

Mastro, TD .

SEXUALLY TRANSMITTED DISEASES, 1997, 24 (03) :142-148

[9] TRANSFER OF PLASMID-MEDIATED AMPICILLIN RESISTANCE FROM HAEMOPHILUS TO NEISSERIA-GONORRHOEAE REQUIRES AN INTERVENING ORGANISM [J].

MCNICOL, PJ ;

ALBRITTON, WL ;

RONALD, AR .

SEXUALLY TRANSMITTED DISEASES, 1986, 13 (03) :145-150

[10] Sequence analysis of the family of penicillinase-producing plasmids of Neisseria gonorrhoeae [J].

Pagotto, F ;

Aman, AT ;

Ng, LK ;

Yeung, KH ;

Brett, M ;

Dillon, JAR .

PLASMID, 2000, 43 (01) :24-34

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