Targeted gene correction: Synthesis and characterization of double-hairpin 2'-O-methyl RNA/DNA chimera oligonucleotides

被引：2
|
作者：
Andrus, A
Vinayak, R
Kuimelis, RG
Mullah, B
机构：
来源：
NUCLEOSIDES & NUCLEOTIDES | 1997年 / 16卷 / 7-9期
关键词：
DNA OLIGONUCLEOTIDE; RNA; NUCLEOSIDES; CHEMISTRY; SUPPORT;
D O I：
10.1080/07328319708006155
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A series of 2'-O-methyl RNA/DNA chimera oligonucleotides were synthesized with a double-hairpin structural motif. Liposome formulated delivery of the chimeras effected targeted, high conversion of mutant alleles in mammalian cell culture. The chimera oligonucleotides were prepared with DNA and 2'-OMe RNA phosphoramidite nucleoside monomers on the ABI 394 synthesizer.
引用
收藏
页码：1183 / 1194
页数：12
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