H-1-NMR study of diamagnetic cytochrome P450cam: assignment of heme resonances and substrate dependance of one cysteinate beta proton

被引：11
|
作者：
Mouro, C
Bondon, A
Simonneaux, G
Jung, C
机构：
[1] UNIV RENNES 1,LAB CHIM ORGANOMET & BIOL,CNRS,UMR 6509,F-35042 RENNES,FRANCE
[2] MAX DELBRUCK CTR MOL MED,D-13125 BERLIN,GERMANY
来源：
FEBS LETTERS | 1997年 / 414卷 / 02期
关键词：
cytochrome P450; H-1-NMR; substrate interaction;
D O I：
10.1016/S0014-5793(97)00995-2
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The H-1-NMR study of diamagnetic cytochrome P450cam Fe-II-CO has been performed for the first time, Chemical shifts of the cysteinate fifth ligand protons and of several heme protons have been assigned through 1- and 2-dimensional spectra at 500 MHz, A substrate dependance has been observed for the resonance of the cysteinate proton detected in the high-field region. (C) 1997 Federation of European Biochemical Societies.
引用
收藏
页码：203 / 208
页数：6
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