Knockdown of Tripartite Motif Containing 24 by Lentivirus Suppresses Cell Growth and Induces Apoptosis in Human Colorectal Cancer Cells

被引：39

作者：

Wang, Jianwei ^{[1
]}

Zhu, Jinhui ^{[1
]}

Dong, Mingjun ^{[2
]}

Yu, Hua ^{[2
]}

Dai, Xiaoyu ^{[2
]}

Li, Keqiang ^{[3
]}

机构：

[1] Zhejiang Univ, Sch Med, Affiliated Hosp 2, Dept Surg Oncol, Hangzhou 310003, Zhejiang, Peoples R China

[2] Ningbo Second Hosp, Dept Anorectal Surg, Ningbo 315010, Zhejiang, Peoples R China

[3] Ningbo Second Hosp, Clin Res Ctr, Key Lab Mol Biol Canc, Ningbo 315010, Zhejiang, Peoples R China

来源：

ONCOLOGY RESEARCH | 2014年 / 22卷 / 01期

基金：

中国国家自然科学基金;

关键词：

TRIM24; Proliferation; Apoptosis; Colorectal cancer; Lentivirus; E3 UBIQUITIN LIGASES; NUCLEAR RECEPTORS; TRIM24; TIF1-ALPHA; BREAST-CANCER; GENE; TRANSCRIPTION; DEGRADATION; PROTEINS; ALPHA; PROGRESSION;

D O I：

10.3727/096504014X14078436005012

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Colorectal cancer remains one of the most common cancers in men and women, and it accounts for a large proportion of cancer-related deaths worldwide. Tripartite motif (TRIM) proteins are a novel class of "single protein RING finger" E3 ubiquitin ligases, which have been shown to be involved in many cancers. The aim of this study was to investigate the potential role of TRIM24 in human colorectal cancer. By using a lentivirus-mediated RNA interference system, we first explored the effect of TRIM24 knockdown on HCT116 cell proliferation and colony formation. Moreover, flow cytometry analysis was used to examine its effects on cell cycle distribution and apoptosis. Our data showed that knockdown of TRIM24 expression in HCT116 cells significantly decreased cell growth due to the induction of apoptosis. Hence, the present study provides evidence that TRIM24 functions as an oncogene in colorectal carcinogenesis.

引用

页码：39 / 45

页数：7

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